Post-injury tendon mechanics are not affected by tamoxifen treatment.

IF 2.8 4区医学 Q3 CELL BIOLOGY

Connective Tissue Research Pub Date : 2023-01-01 Epub Date: 2022-07-11 DOI:10.1080/03008207.2022.2097907

Zakary M Beach, Ashley K Fung, Stephanie N Weiss, Louis J Soslowsky

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引用次数: 2

Abstract

Purpose: A growing interest in the mechanisms that govern tendon healing has resulted in the develop-ment of tools, such as the tamoxifen-inducible mouse knockdown model, to address these questions. However, tamoxifen is a selective estrogen receptor modulator and may interfere with the tendon healing process. The objective of this study was to evaluate the effects of tamoxifen on post-injury tendon mechanics in wild-type mice.

Methods: The mice underwent treatment at the time of injury using an established mouse injury model and the injured tendons were evaluated 3 weeks post-injury. The treatment contained tamoxifen suspended in corn oil and was compared to a treatment with only corn oil, as well as mice with no treatment. Tendons were evaluated by measuring the quasi-static and viscoelastic mechanics, collagen fiber realignment, cellularity, and nuclear morphology.

Results: Mechanical testing of the tendons post-injury revealed no changes to viscoelastic mechanics, quasi-static mechanics, or collagen realignment during loading after tamoxifen treatment with the dosage regimen utilized (three daily injections of 4.5 mg/40 g body weight). Additionally, histological analysis revealed no changes to cellularity or cell nuclear shape.

Conclusion: Overall, this study revealed that tamoxifen treatment at the time of tendon injury did not result in changes to tendon mechanics or the histological parameters at 3 weeks post-injury.

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他莫昔芬治疗不会影响受伤后肌腱的力学结构。

目的：人们对肌腱愈合的机制越来越感兴趣，因此开发了一些工具，如他莫昔芬诱导的小鼠基因敲除模型，以解决这些问题。然而，他莫昔芬是一种选择性雌激素受体调节剂，可能会干扰肌腱愈合过程。本研究的目的是评估他莫昔芬对野生型小鼠损伤后肌腱力学的影响：方法：使用已建立的小鼠损伤模型，在小鼠受伤时对其进行治疗，并在受伤后 3 周对受伤肌腱进行评估。治疗中使用了悬浮在玉米油中的他莫昔芬，并与仅使用玉米油的治疗方法和未使用任何治疗方法的小鼠进行了比较。通过测量准静力学和粘弹性力学、胶原纤维重新排列、细胞度和核形态对肌腱进行了评估：结果：对受伤后肌腱进行的力学测试表明，使用他莫昔芬治疗后（每天注射三次，每次 4.5 毫克/40 克体重），肌腱在加载过程中的粘弹性力学、准静态力学或胶原纤维重新排列均无变化。此外，组织学分析表明，细胞度或细胞核形状没有发生变化：总之，这项研究表明，在肌腱损伤时进行他莫昔芬治疗不会导致肌腱力学或损伤后 3 周的组织学参数发生变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Connective Tissue Research 生物-细胞生物学

CiteScore

6.60

自引率

3.40%

发文量

审稿时长

2 months

期刊介绍： The aim of Connective Tissue Research is to present original and significant research in all basic areas of connective tissue and matrix biology. The journal also provides topical reviews and, on occasion, the proceedings of conferences in areas of special interest at which original work is presented. The journal supports an interdisciplinary approach; we present a variety of perspectives from different disciplines, including Biochemistry Cell and Molecular Biology Immunology Structural Biology Biophysics Biomechanics Regenerative Medicine The interests of the Editorial Board are to understand, mechanistically, the structure-function relationships in connective tissue extracellular matrix, and its associated cells, through interpretation of sophisticated experimentation using state-of-the-art technologies that include molecular genetics, imaging, immunology, biomechanics and tissue engineering.