Cytogenetic characterization of solitary wasp Ancistrocerus flavomarginatus (Brèthes, 1906) (Hymenoptera, Vespidae) with insights into the chromosomal evolution in the genus.

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Genome Pub Date : 2023-03-01 DOI:10.1139/gen-2022-0095
Mara Garcia Tavares, Gisele Amaro Teixeira
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引用次数: 0

Abstract

Cytogenetic studies have enabled the characterization of the chromosomal macrostructure and microstructure and have contributed to the understanding of the evolution of wasp karyotypes. However, studies on Eumeninae solitary wasps are scarce. In this study, we characterized the karyotype of Ancistrocerus flavomarginatus (Brèthes, 1906) and compared it with previous data from other Ancistrocerus (Wesmael, 1836) species to shed light on the chromosomal diversity of the genus. A chromosome number of 2n = 24 in females and n = 12 in males was observed. Comparing the A. flavomarginatus karyotype with that of another Ancistrocerus species showed variations in the morphology of some chromosomal pairs. The presence of two larger chromosome pairs, almost entirely heterochromatic, and the predominance of subtelocentric chromosomes with heterochromatic short arms in A. flavomarginatus support the occurrence of fissions in Ancistrocerus. A single site of ribosomal genes was observed in A. flavomarginatus, in addition to a size polymorphism of these rDNA clusters between the homologues of some analyzed females. This polymorphism may originate from duplications/deletions due to unequal crossing-over or amplification via transposable elements. The (GA)15 microsatellite is located exclusively in euchromatic regions. Our data show that different rearrangements seem to shape chromosomal evolution in Ancistrocerus species.

黄斑孤蜂(Ancistrocerus flavomarginatus, br, 1906)(膜翅目,蜂科)的细胞遗传学特征及其属染色体进化的认识。
细胞遗传学研究使染色体宏观结构和微观结构的表征成为可能,并有助于理解黄蜂核型的进化。然而,对独居胡蜂的研究却很少。本研究对Ancistrocerus flavomarginatus (br thes, 1906)的核型进行了鉴定,并与其他Ancistrocerus (Wesmael, 1836)种的资料进行了比较,以阐明该属的染色体多样性。雌性染色体数为2n = 24,雄性染色体数为n = 12。将黄尾猿核型与另一种蛇尾猿核型进行比较,发现某些染色体对的形态存在差异。A. flavomarginatus中两对较大的几乎完全异色的染色体的存在,以及亚远心短臂异色染色体的优势,支持了Ancistrocerus分裂的发生。黄颡鱼的核糖体基因只有一个位点,而且这些rDNA簇的大小在一些雌性同系物之间存在多态性。这种多态性可能源于不平等交叉或转座元件扩增造成的重复/缺失。(GA)15微卫星仅位于正色区域。我们的数据显示,不同的重排似乎塑造了蛇尾龙物种的染色体进化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genome
Genome 生物-生物工程与应用微生物
CiteScore
5.30
自引率
3.20%
发文量
42
审稿时长
6-12 weeks
期刊介绍: Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.
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