Histopathological examination of the protective effect of intense exercise in apoptotic germ cell damage due to diabetes.

IF 1.1 4区医学 Q3 SURGERY

Acta cirurgica brasileira Pub Date : 2023-01-01 DOI:10.1590/acb381423

Veysel Toprak, Senem Alkan Akalın, Ece Öcal, Yunus Çavuş, İlhan Özdemir

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引用次数: 2

Abstract

Purpose: The aim of this study was to determine the protective and antioxidative effects of intensive exercise on streptozotocin (STZ)-induced testicular damage, apoptotic spermatognial cells death, and oxidative stress.

Methods: 36 male Sprague Dawley rats were divided into three groups: control, diabetes, and diabetes+intensive exercise (IE) groups. Testicular tissues were examined histopathologically and antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA) activity, as well as serum testosterone level, were measured.

Results: Seminiferous tubules and germ cells were found to be better in the testis tissue of the intense exercise group than in the diabetes group. Diabetes suppressed antioxidant enzymes CAT, SOD, GPx and testosterone levels were significantly decreased, and increased MDA level in the diabetic group compared to diabetes+IE group (p < 0.001). Following four weeks of treatment, intensive exercise improved the antioxidant defense, significantly decreased MDA activity, and increased testosterone levels in testicular tissue in the diabetic group compared to diabetes+IE group (p < 0.01).

Conclusions: STZ-induced diabetes causes damage to the testis tissue. In order to prevent these damages, exercise practice has become very popular nowadays. In present study, our intensive exercise protocol, histological, and biochemical analysis of the effect of diabetes on the testicular tissues is shown.

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高强度运动对糖尿病所致生殖细胞凋亡损伤保护作用的组织病理学观察。

目的:研究高强度运动对链脲佐菌素(STZ)诱导的睾丸损伤、凋亡性精子细胞死亡和氧化应激的保护作用和抗氧化作用。方法:将36只雄性Sprague Dawley大鼠分为对照组、糖尿病组和糖尿病+强化运动组(IE)。对大鼠睾丸组织进行组织病理学检查，测定过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)、丙二醛(MDA)等抗氧化酶活性及血清睾酮水平。结果:高强度运动组睾丸组织中精管和生殖细胞的变化明显优于糖尿病组。与糖尿病+IE组相比，糖尿病组抗氧化酶CAT、SOD、GPx、睾酮水平显著降低，MDA水平显著升高(p < 0.001)。治疗4周后，与糖尿病+IE组相比，高强度运动增强了糖尿病组的抗氧化防御能力，显著降低了MDA活性，睾丸激素水平升高(p < 0.01)。结论:stz诱导的糖尿病可引起睾丸组织损伤。为了防止这些损害，锻炼已经变得非常流行。在本研究中，我们的强化运动方案，糖尿病对睾丸组织的影响的组织学和生化分析显示。

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