Comparative analysis of neurofilament light chain levels in the serum and cerebrospinal fluid in rats subjected to partial sciatic nerve ligation.

IF 0.9 4区医学 Q4 PATHOLOGY

Journal of Toxicologic Pathology Pub Date : 2023-04-01 DOI:10.1293/tox.2022-0110

Tomoya Sano, Yasushi Masuda, Hironobu Yasuno, Takeshi Watanabe, Tadahiro Shinozawa

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引用次数: 1

Abstract

Neurofilament light chain (NfL) has recently been used as a biomarker of neurodegeneration. Although cerebrospinal fluid (CSF) NfL levels are hypothesized to affect blood NfL levels, whether blood NfL levels change independently of the CSF during peripheral nerve injury remains unclear. Thus, we evaluated the nervous tissues histopathology and serum and CSF NfL levels in partial sciatic nerve-ligated rats at 6 h and one, three, or seven days after the surgery. Sciatic and tibial nerve fiber damage was observed at 6 h after the surgery, and peaked at three days postoperatively. The serum NfL levels peaked 6 h to one day after ligation, but they tended to return to the normal seven days after ligation. However, the CSF NfL levels were unchanged throughout the study period. In conclusion, the comparative evaluation of serum and CSF NfL levels can provide useful information as biomarkers of nerve tissue damage and its distribution.

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坐骨神经部分结扎大鼠血清和脑脊液中神经丝轻链水平的比较分析。

神经丝轻链(NfL)最近被用作神经变性的生物标志物。虽然脑脊液(CSF) NfL水平被假设影响血液NfL水平，但外周血神经损伤时血液NfL水平是否独立于CSF变化尚不清楚。因此，我们评估了部分坐骨神经结扎大鼠在术后6小时、1天、3天或7天的神经组织病理学、血清和脑脊液NfL水平。术后6 h观察到坐骨和胫骨神经纤维损伤，术后3 d达到峰值。结扎后6 h ~ 1天血清NfL水平达到峰值，结扎后7天趋于正常。然而，CSF NfL水平在整个研究期间没有变化。综上所述，血清和脑脊液NfL水平的比较评估可以作为神经组织损伤及其分布的生物标志物提供有用的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Toxicologic Pathology PATHOLOGY-TOXICOLOGY

CiteScore

2.10

自引率

16.70%

发文量

审稿时长

>12 weeks

期刊介绍： JTP is a scientific journal that publishes original studies in the field of toxicological pathology and in a wide variety of other related fields. The main scope of the journal is listed below. Administrative Opinions of Policymakers and Regulatory Agencies Adverse Events Carcinogenesis Data of A Predominantly Negative Nature Drug-Induced Hematologic Toxicity Embryological Pathology High Throughput Pathology Historical Data of Experimental Animals Immunohistochemical Analysis Molecular Pathology Nomenclature of Lesions Non-mammal Toxicity Study Result or Lesion Induced by Chemicals of Which Names Hidden on Account of the Authors Technology and Methodology Related to Toxicological Pathology Tumor Pathology; Neoplasia and Hyperplasia Ultrastructural Analysis Use of Animal Models.