Comparison of the Effects of Tissue Processing on the Physicochemical Properties of Bone Allografts.

IF 1.7 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE
Elnaz Ajami, Cong Fu, Sun Jin Park, Xuesong Wang, Hai Bo Wen
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引用次数: 0

Abstract

Purpose: To address the hypothesis that the tissue processing methods of solvent dehydration and freeze-drying would differentially affect the physicochemical characteristics of four commercially available bone allografts and the adhesion and differentiation of human bone marrow-derived mesenchymal stromal cells (hBMSCs) on such substrates in vitro. Materials and Methods: The surface morphology, surface area, and elemental composition of four commercially available cancellous bone allografts were examined using SEM, Brunauer-Emmett-Teller (BET) gas adsorption, and inductively coupled plasma (ICP) analyses. SEM was also employed to compare the allograft surfaces to that of human bone exposed by in vitro osteoclastic resorption. The allografts were seeded with hBMSCs, and the number of adhered cells was assessed at 3 and 7 days. Alkaline phosphatase (ALP) activity was quantified as a measure of osteogenic differentiation after 21 days. Results: Marked differences were seen between the physicochemical characteristics of the solvent-dehydrated and freeze-dried allografts, as well as between their resulting bone microarchitectures and that of osteoclast-resorbed human bone. Increased hBMSC adhesion and differentiation were observed on the solvent-dehydrated allografts compared to freeze-dried allografts, which suggests a higher putative osteogenic potential. The latter was attributed to better preservation of the bone collagen microarchitecture integrity, which may provide not only a more complex substrate architecture, but also a more favorable microenvironment to allow nutrients and oxygen to flow to the adhered cells. Conclusion: Commercially available cancellous bone allografts significantly differ in their physicochemical characteristics, stemming from differences in tissue processing and sterilization methods undertaken by tissue banks. These differences impact the response of MSCs in vitro and may alter the biologic performance of the grafts in vivo. Therefore, it is important to consider these characteristics when choosing a bone substitute for clinical application, as the physicochemical properties of the grafts play a crucial role in their interactions with the biologic environment and subsequent incorporation into the native bone.

组织处理对同种异体骨移植物理化性质影响的比较。
目的:探讨溶剂脱水和冷冻干燥两种组织处理方法对四种市售同种异体骨移植物的理化特性以及人骨髓间充质基质细胞(hBMSCs)在体外的粘附和分化的影响。材料和方法:采用扫描电镜(SEM)、布鲁诺尔-埃米特-泰勒(BET)气体吸附和电感耦合等离子体(ICP)分析,对四种市售的同种异体松质骨移植物的表面形貌、表面积和元素组成进行了研究。扫描电镜还比较了同种异体移植骨与体外破骨吸收暴露的人骨的表面。在同种异体移植物中植入hBMSCs,在第3天和第7天评估粘附细胞的数量。21天后,定量测定碱性磷酸酶(ALP)活性作为成骨分化的指标。结果:溶剂脱水和冻干同种异体骨的理化特性、骨微结构与破骨细胞吸收的人骨存在明显差异。与冻干同种异体移植物相比,溶剂脱水同种异体移植物的hBMSC粘附和分化增强,这表明其具有更高的成骨潜力。后者归因于更好地保存了骨胶原微结构的完整性,这可能不仅提供了更复杂的底物结构,而且提供了更有利的微环境,使营养物质和氧气流向粘附的细胞。结论:市售同种异体松质骨移植物的理化特性存在显著差异,这是由于组织库的组织处理和灭菌方法不同所致。这些差异影响了MSCs在体外的反应,并可能改变移植物在体内的生物学性能。因此,在选择临床应用的骨替代物时,考虑这些特征是很重要的,因为移植物的物理化学性质在其与生物环境的相互作用以及随后与天然骨的结合中起着至关重要的作用。
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来源期刊
CiteScore
3.30
自引率
5.00%
发文量
115
审稿时长
6 months
期刊介绍: Edited by Steven E. Eckert, DDS, MS ISSN (Print): 0882-2786 ISSN (Online): 1942-4434 This highly regarded, often-cited journal integrates clinical and scientific data to improve methods and results of oral and maxillofacial implant therapy. It presents pioneering research, technology, clinical applications, reviews of the literature, seminal studies, emerging technology, position papers, and consensus studies, as well as the many clinical and therapeutic innovations that ensue as a result of these efforts. The editorial board is composed of recognized opinion leaders in their respective areas of expertise and reflects the international reach of the journal. Under their leadership, JOMI maintains its strong scientific integrity while expanding its influence within the field of implant dentistry. JOMI’s popular regular feature "Thematic Abstract Review" presents a review of abstracts of recently published articles on a specific topical area of interest each issue.
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