Comparison of three fluids for calibration of the new Periotron® 8010.

IF 2.2 3区 医学
M Fernández-Reyes, C-F Márquez-Arrico, F-J Silvestre, L Perea-Galera, J Silvestre-Rangil, M Rocha
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引用次数: 0

Abstract

Background: The aim of the present study was to calibrate the Periotron® model 8010 with volumes of three different fluids (distilled water, serum, and saliva) and to identify which of the three is the most reliable, feasible, and reproducible for routine calibration.

Material and methods: A total of 450 samples of Periopaper® were divided into three groups (150 each per group): distilled water, serum matrix and saliva. A calibration curve was run with 0.25, 0.50, 0.75, 1.00 and 1.25 µl of each of the fluids, and the results were determined in Periotron units (PU). Statistical analysis was performed with one-way ANOVA followed by Bonferroni's post hoc test and a linear equation.

Results: Distilled water presented the lowest levels of PU at all volumes, while serum showed the highest levels at high volumes. Linear regression equations rendered similar slopes for saliva and distilled water, while serum was statistically different. Saliva presented a reproduction percentage of 99.7%, which indicated better accuracy and precision than serum and distilled water.

Conclusions: Saliva is more reliable and accurate than water or serum for the purpose of calibration of the Periotron® model 8010, though it shares drawbacks with serum. Distilled water is more easily available and does not require any additional procedure, in addition to producing a similar slope to saliva and a smaller deviation from the media than serum.

用于校准新型Periotron®8010的三种流体的比较。
背景:本研究的目的是用三种不同液体(蒸馏水、血清和唾液)的体积校准Periotron®8010型,并确定这三种液体中哪一种最可靠、可行和可重复用于常规校准。材料和方法:总共450份Periopaper®样本被分为三组(每组150份):蒸馏水、血清基质和唾液。使用0.25、0.50、0.75、1.00和1.25µl的每种流体运行校准曲线,并以Periotron单位(PU)测定结果。统计分析采用单因素方差分析,然后采用Bonferroni事后检验和线性方程。结果:蒸馏水在所有体积下PU含量最低,而血清在高体积下PU水平最高。线性回归方程显示唾液和蒸馏水的斜率相似,而血清在统计学上不同。唾液的繁殖率为99.7%,其准确度和精密度优于血清和蒸馏水。结论:在校准Periotron®8010型时,唾液比水或血清更可靠、更准确,尽管它与血清有共同的缺点。蒸馏水更容易获得,不需要任何额外的程序,除了产生与唾液相似的斜率和与培养基相比更小的偏差外。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.50
自引率
0.00%
发文量
52
期刊介绍: 1. Oral Medicine and Pathology: Clinicopathological as well as medical or surgical management aspects of diseases affecting oral mucosa, salivary glands, maxillary bones, as well as orofacial neurological disorders, and systemic conditions with an impact on the oral cavity. 2. Oral Surgery: Surgical management aspects of diseases affecting oral mucosa, salivary glands, maxillary bones, teeth, implants, oral surgical procedures. Surgical management of diseases affecting head and neck areas. 3. Medically compromised patients in Dentistry: Articles discussing medical problems in Odontology will also be included, with a special focus on the clinico-odontological management of medically compromised patients, and considerations regarding high-risk or disabled patients. 4. Implantology 5. Periodontology
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