The effect of extracellular polymeric substances (EPS) of iron-oxidizing bacteria (Ochrobactrum EEELCW01) on mineral transformation and arsenic (As) fate

IF 6.9 Q1 Environmental Science
Chuan Wu , Yueru Chen , Ziyan Qian , Hongren Chen , Waichin Li , Qihou Li , Shengguo Xue
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引用次数: 8

Abstract

Extracellular polymeric substances (EPS) are an important medium for communication and material exchange between iron-oxidizing bacteria and the external environment and could induce the iron (oxyhydr) oxides production which reduced arsenic (As) availability. The main component of EPS secreted by iron-oxidizing bacteria (Ochrobactrum EEELCW01) was composed of polysaccharides (150.76-165.33 mg/g DW) followed by considerably smaller amounts of proteins (12.98–16.12 mg/g DW). Low concentrations of As (100 or 500 µmol/L) promoted the amount of EPS secretion. FTIR results showed that EPS was composed of polysaccharides, proteins, and a miniscule amount of nucleic acids. The functional groups including -COOH, -OH, -NH, -C=O, and -C-O played an important role in the adsorption of As. XPS results showed that As was bound to EPS in the form of As3+. With increasing As concentration, the proportion of As3+ adsorbed on EPS increased. Ferrihydrite with a weak crystalline state was only produced in the system at 6 hr during the mineralization process of Ochrobactrum sp. At day 8, the minerals were composed of goethite, galena, and siderite. With the increasing mineralization time, the main mineral phases were transformed from weakly crystalline hydrous iron ore into higher crystallinity siderite (FeCO3) or goethite (α-FeOOH), and the specific surface area and active sites of minerals were reduced. It can be seen from the distribution of As elements that As is preferentially adsorbed on the edges of iron minerals. This study is potential to understand the biomineralization mechanism of iron-oxidizing bacteria and As remediation in the environment.

铁氧化菌(Ochrobactrum EEELCW01)胞外聚合物(EPS)对矿物转化和砷(As)命运的影响
细胞外聚合物(EPS)是铁氧化细菌与外部环境之间交流和物质交换的重要介质,可诱导铁(羟基)氧化物的产生,从而降低砷(As)的可用性。铁氧化细菌(Ochrobactrum EEELCW01)分泌的EPS的主要成分由多糖(150.76-165.33毫克/克DW)和少量蛋白质(12.98-16.12毫克/克DW)组成。低浓度的As(100或500µmol/L)促进了EPS的分泌量。FTIR结果表明,EPS由多糖、蛋白质和少量核酸组成。COOH、-OH、-NH、-C=O和-C-O等官能团在As的吸附过程中起着重要作用。XPS结果表明,As以As3+的形式与EPS结合。随着As浓度的增加,As3+在EPS上的吸附比例增加。在Ochrobactrum sp.的矿化过程中,仅在6小时时在系统中产生了具有弱结晶状态的铁素体。在第8天,矿物由针铁矿、方铅矿和菱铁矿组成。随着矿化时间的增加,主要矿物相由弱结晶含水铁矿转变为结晶度较高的菱铁矿(FeCO3)或针铁矿(α-FeOOH),矿物的比表面积和活性位点减少。从As元素的分布可以看出,As优先吸附在铁矿物的边缘。本研究有助于了解铁氧化菌的生物矿化机制和砷在环境中的修复作用。
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来源期刊
Journal of environmental sciences
Journal of environmental sciences Environmental Science (General)
CiteScore
12.80
自引率
0.00%
发文量
0
审稿时长
17 days
期刊介绍: Journal of Environmental Sciences is an international peer-reviewed journal established in 1989. It is sponsored by the Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, and it is jointly published by Elsevier and Science Press. It aims to foster interdisciplinary communication and promote understanding of significant environmental issues. The journal seeks to publish significant and novel research on the fate and behaviour of emerging contaminants, human impact on the environment, human exposure to environmental contaminants and their health effects, and environmental remediation and management. Original research articles, critical reviews, highlights, and perspectives of high quality are published both in print and online.
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