Analysis of Th-cell subsets in local and systemic environments from experimental periodontitis rats.

IF 2.8 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Molecular Oral Microbiology Pub Date : 2023-04-01 DOI:10.1111/omi.12376

Yun Yuan, Hongming Zhang, Qinhua Gu, Xinrui Xu, Runping Yu, Hui Huang

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引用次数: 3

Abstract

Objectives: The objective of this study was to explore the effect of periodontitis on Th-cell subsets in local and systemic environments.

Methods: A total of 32 male Sprague-Dawley rats were randomly divided into periodontitis and control groups. Silk ligatures were applied to the mandibular first (M1) molars in the periodontitis group. Inflammation and alveolar bone loss around the M1 molars were analyzed by histological staining and microcomputed tomography. The mRNA expression of interferon-γ (IFN-γ), interleukin 4 (IL-4), IL-17, and IL-10 in the gingiva was measured by qRT-PCR. The proportions of Th1, Th2, Th17, and Treg cells in the submandibular lymph nodes, peripheral blood, and jaw bone marrow were tested using flow cytometry.

Results: More inflammatory cells and alveolar bone resorption were found in the periodontitis group, with upregulated mRNA expression of IFN-γ, IL-17, and IL-10. The proportion of Th1 and Th17 cells was significantly elevated in submandibular lymph nodes, and the proportion of Th1, Th2, and Th17 cells was significantly elevated in peripheral blood, while the proportion of Th1, Th17, and Treg cells was significantly elevated in jaw bone marrow in the periodontitis group.

Conclusion: This study suggests that periodontitis affects the differentiation of Th-cell subsets in both local and systemic environments, resulting in an increased proportion of proinflammatory cells.

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实验性牙周炎大鼠局部和全身环境中th细胞亚群的分析。

目的:本研究的目的是探讨牙周炎对局部和全身环境中th细胞亚群的影响。方法:32只雄性Sprague-Dawley大鼠随机分为牙周炎组和对照组。牙周炎组采用丝线结扎下颌第一磨牙(M1)。通过组织染色和显微计算机断层扫描分析M1磨牙周围的炎症和牙槽骨丢失。采用qRT-PCR方法检测牙龈组织中干扰素-γ (IFN-γ)、白细胞介素4 (IL-4)、IL-17、IL-10 mRNA的表达。流式细胞术检测大鼠下颌骨淋巴结、外周血和颌骨骨髓中Th1、Th2、Th17和Treg细胞的比例。结果:牙周炎组炎症细胞增多，牙槽骨吸收增多，IFN-γ、IL-17、IL-10 mRNA表达上调。牙周炎组下颌骨淋巴结中Th1、Th17细胞比例显著升高，外周血中Th1、Th2、Th17细胞比例显著升高，颌骨骨髓中Th1、Th17、Treg细胞比例显著升高。结论:本研究提示牙周炎影响局部和全身环境中th细胞亚群的分化，导致促炎细胞比例增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Oral Microbiology DENTISTRY, ORAL SURGERY & MEDICINE-MICROBIOLOGY

CiteScore

6.50

自引率

5.40%

发文量

审稿时长

>12 weeks

期刊介绍： Molecular Oral Microbiology publishes high quality research papers and reviews on fundamental or applied molecular studies of microorganisms of the oral cavity and respiratory tract, host-microbe interactions, cellular microbiology, molecular ecology, and immunological studies of oral and respiratory tract infections. Papers describing work in virology, or in immunology unrelated to microbial colonization or infection, will not be acceptable. Studies of the prevalence of organisms or of antimicrobials agents also are not within the scope of the journal. The journal does not publish Short Communications or Letters to the Editor. Molecular Oral Microbiology is published bimonthly.