Validation of a New PCR-Based Screening Method for Prevention of Serratia marcescens Outbreaks in the Neonatal Intensive Care Unit.

IF 2.6 3区医学 Q1 PEDIATRICS

Neonatology Pub Date : 2023-01-01 DOI:10.1159/000526836

Lina K Sciesielski, Luisa K M Osang, Nicole Dinse, Anna Weber, Christoph Bührer, Axel Kola, Christof Dame

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引用次数: 0

Abstract

Background: Serratia marcescens may cause severe nosocomial infections, mostly in very low birth weight infants. Since S. marcescens exhibits by far the highest adjusted incidence rate for horizontal transmission, it can cause complex outbreak situations in neonatal intensive care units.

Objective: The aim of this study was to establish a fast and highly sensitive colonization screening for prompt cohorting and barrier nursing strategies.

Methods: A probe-based duplex PCR assay targeting the 16S rRNA gene of S. marcescens was developed and validated by using 36 reference strains, 14 S. marcescens outbreak- and nonoutbreak isolates, defined by epidemiological linkage and molecular typing, and applied in 1,347 clinical specimens from 505 patients.

Results and conclusions: The novel PCR assay proved to be highly specific and had an in vitro sensitivity of 100 gene copies per reaction (∼15 bacteria). It showed a similar (in laryngeal/tracheal specimens) or even higher (in rectal/stoma swabs) in vivo sensitivity in comparison to routine microbial culture and was much quicker (<24 h vs. 2 days). By combining different oligonucleotide primers, there was robust detection of genetic variants of S. marcescens strains. PCR inhibition was low (1.6%) and observed with rectal swabs only. Cohort analysis illustrated applicability of the PCR assay as a quick tool to prevent outbreak scenarios by allowing rapid decisions on cohorting and barrier nursing. In summary, this novel molecular screening for colonization by S. marcescens is specific, highly sensitive, and substantially accelerates detection.

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一种新的基于聚合酶链反应的筛查方法在新生儿重症监护室预防粘质沙雷菌爆发的验证。

背景:粘质沙雷菌可引起严重的医院感染，主要发生在极低出生体重儿中。由于粘质葡萄球菌在水平传播方面的调整后发病率最高，因此它可在新生儿重症监护病房引起复杂的暴发情况。目的:本研究的目的是建立一种快速、高灵敏度的菌落筛选方法，用于快速队列和屏障护理策略。方法:采用36株参比菌株、14株黏质葡萄球菌爆发株和14株非爆发株，采用流行病学连锁和分子分型方法，建立了针对黏质葡萄球菌16S rRNA基因的探针双链PCR检测方法，并应用于505例患者的1347份临床标本。结果和结论:新的PCR检测被证明是高度特异性的，并且每个反应(~ 15个细菌)具有100个基因拷贝的体外敏感性。与常规微生物培养相比，它显示出相似的(喉/气管标本)甚至更高的(直肠/口拭子)体内敏感性，并且更快(

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来源期刊

Neonatology 医学-小儿科

CiteScore

0.60

自引率

4.00%

发文量

审稿时长

6-12 weeks

期刊介绍： This highly respected and frequently cited journal is a prime source of information in the area of fetal and neonatal research. Original papers present research on all aspects of neonatology, fetal medicine and developmental biology. These papers encompass both basic science and clinical research including randomized trials, observational studies and epidemiology. Basic science research covers molecular biology, molecular genetics, physiology, biochemistry and pharmacology in fetal and neonatal life. In addition to the classic features the journal accepts papers for the sections Research Briefings and Sources of Neonatal Medicine (historical pieces). Papers reporting results of animal studies should be based upon hypotheses that relate to developmental processes or disorders in the human fetus or neonate.