Air pollution in Sarajevo, Bosnia and Herzegovina, assessed by plant comet assay.

IF 2.5 4区 医学 Q3 GENETICS & HEREDITY
Mutagenesis Pub Date : 2023-02-03 DOI:10.1093/mutage/geac022
Mujo Hasanovic, Tamara Cetkovic, Bertrand Pourrut, Lejla Caluk Klacar, Maida Hadzic Omanovic, Adaleta Durmic-Pasic, Sanin Haveric, Anja Haveric
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引用次数: 3

Abstract

Bosnia and Herzegovina (B&H) is among the European countries with the highest rate of air pollution-related death cases and the poorest air quality. The main causes are solid fuel consumption, traffic, and the poorly developed or implemented air pollution reduction policies. In addition, the city of Sarajevo, the capital of B&H, suffers temperature inversion episodes in autumn/winter months, which sustain air pollution. Human biomonitoring studies may be confounded by the lifestyle of subjects or possible metabolic alterations. Therefore, this study aimed to evaluate Ligustrum vulgare L. as a model for air pollution monitoring by measuring DNA damage at one rural and two urban sites. DNA damage was measured as tail intensity (TI) in L. vulgare leaves, considering seasonal, sampling period, leaf position and staging, and spatial (urban versus rural) variation. Effects of COVID-19 lockdown on TI were assessed by periodical monitoring at one of the selected sites, while in-house grown L. vulgare plants were used to test differences between outdoor and indoor air pollution effects for the same sampling period. Significantly higher TI was generally observed in leaves collected in Campus in December 2020 and 2021 compared with March (P < 0.0001). Outer and adult leaves showed higher TI values, except for the rural site where no differences for these categories were found. Leaves collected in the proximity of the intensive traffic showed significantly higher TI values (P < 0.001), regardless of the sampling period and the stage of growth. In regards to the COVID-19 lockdown, higher TI (P < 0.001) was registered in December 2020, after the lockdown period, than in periods before COVID-19 outbreak or immediately after the lockdown in 2020. This also reflects mild air pollution conditions in summer. TI values for the in-house grown leaves were significantly lower compared to those in situ. Results showed that L. vulgare may present a consistent model for the air pollution biomonitoring but further studies are needed to establish the best association between L. vulgare physiology, air quality data, and air pollution effects.

用植物彗星测定法评价波斯尼亚和黑塞哥维那萨拉热窝的空气污染。
波斯尼亚和黑塞哥维那是与空气污染有关的死亡率最高、空气质量最差的欧洲国家之一。主要原因是固体燃料消耗,交通,以及不完善或执行的空气污染减少政策。此外,波斯尼亚和黑塞哥维那的首都萨拉热窝市在秋冬月份出现逆温现象,造成空气污染。人体生物监测研究可能会被受试者的生活方式或可能的代谢改变所混淆。因此,本研究旨在通过测量一个农村和两个城市地点的DNA损伤来评价女贞子(Ligustrum vulgare L.)作为空气污染监测的模型。考虑季节、采样周期、叶片位置和分期以及城市和农村的空间差异,以尾强度(TI)来测量L. vulgare叶片的DNA损伤。通过在选定的一个站点进行定期监测来评估COVID-19封锁对TI的影响,同时使用室内种植的L. vulgare植物来测试相同采样周期内室外和室内空气污染影响的差异。与3月份相比,2020年12月和2021年12月校园采集的叶片TI普遍显著升高(P < 0.0001)。外叶和成叶的TI值较高,除了农村地区,这些类别没有发现差异。无论采样周期和生长阶段如何,靠近交通密集地区采集的叶片TI值均显著高于其他地区(P < 0.001)。在新冠肺炎封城后,2020年12月的TI (P < 0.001)高于新冠肺炎疫情爆发前或2020年封城后的时期。这也反映了夏季空气污染程度较轻。室内生长叶片的TI值明显低于原位叶片。结果表明,普通乳酸菌可能为空气污染的生物监测提供了一致的模型,但需要进一步的研究来建立普通乳酸菌生理、空气质量数据和空气污染效应之间的最佳关联。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Mutagenesis
Mutagenesis 生物-毒理学
CiteScore
5.90
自引率
3.70%
发文量
22
审稿时长
6-12 weeks
期刊介绍: Mutagenesis is an international multi-disciplinary journal designed to bring together research aimed at the identification, characterization and elucidation of the mechanisms of action of physical, chemical and biological agents capable of producing genetic change in living organisms and the study of the consequences of such changes.
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