Comparison of laser speckle contrast imaging with laser Doppler perfusion imaging for tissue perfusion measurement

IF 1.9 4区医学 Q3 HEMATOLOGY

Microcirculation Pub Date : 2022-12-15 DOI:10.1111/micc.12795

Goksel Guven, Annemieke Dijkstra, Tjallingius Martijn Kuijper, Nicole Trommel, Margriet Elisabeth van Baar, Arzu Topeli, Can Ince, Cornelis Hendrik van der Vlies

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引用次数: 3

Abstract

Objective

Laser-based tissue perfusion monitoring techniques have been increasingly used in animal and human research to assess blood flow. However, these techniques use arbitrary units, and knowledge about their comparability is scarce. This study aimed to model the relationship between laser speckle contrast imaging (LSCI) and laser Doppler perfusion imaging (LDPI), for measuring tissue perfusion over a wide range of blood flux values.

Methods

Fifteen healthy volunteers (53% female, median age 29 [IQR 22–40] years) were enrolled in this study. We performed iontophoresis with sodium nitroprusside on the forearm to induce regional vasodilation to increase skin blood flux. Besides, a stepwise vascular occlusion was applied on the contralateral upper arm to reduce blood flux. Both techniques were compared using a linear mixed model analysis.

Results

Baseline blood flux values measured by LSCI were 33 ± 6.5 arbitrary unit (AU) (Coefficient of variation [CV] = 20%) and by LDPI 60 ± 11.5 AU (CV = 19%). At the end of the iontophoresis protocol, the regional blood flux increased to 724 ± 412% and 259 ± 87% of baseline measured by LDPI and LSCI, respectively. On the other hand, during the stepwise vascular occlusion test, the blood flux reduced to 212 ± 40% and 412 ± 177% of its baseline at LDPI and LSCI, respectively. A strong correlation was found between the LSCI and LDPI instruments at increased blood flux with respect to baseline skin blood flux; however, the correlation was weak at reduced blood flux with respect to baseline.

Discussion

LSCI and LDPI instruments are highly linear for blood flux higher than baseline skin blood flux; however, the correlation decreased for blood flux lower than baseline. This study's findings could be a basis for using LSCI in specific patient populations, such as burn care.

Abstract Image

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激光散斑造影与激光多普勒灌注成像在组织灌注测量中的比较

目的激光组织灌注监测技术已越来越多地应用于动物和人体研究中，以评估血流。然而，这些技术使用的是任意单位，关于它们的可比性的知识很少。本研究旨在模拟激光散斑对比成像(LSCI)和激光多普勒灌注成像(LDPI)之间的关系，用于测量大范围血流量值下的组织灌注。方法选取15名健康志愿者，其中女性53%，中位年龄29岁[IQR 22-40]。我们用硝普钠离子导入前臂，诱导局部血管舒张，增加皮肤血流量。此外，在对侧上臂进行逐步血管闭塞以减少血流量。采用线性混合模型分析对两种技术进行比较。结果LSCI测定的基线血通量值为33±6.5任意单位(AU)(变异系数[CV] = 20%)， LDPI测定的基线血通量值为60±11.5 AU (CV = 19%)。在离子透入方案结束时，局部血通量分别增加到LDPI和LSCI测量的基线的724±412%和259±87%。另一方面，在逐步血管闭塞试验中，LDPI和LSCI时的血通量分别下降到基线的212±40%和412±177%。与基线皮肤血流量相比，LSCI和LDPI仪器在血流量增加方面存在很强的相关性;然而，相对于基线，在血流量降低时相关性较弱。LSCI和LDPI仪器的血流量高于基线皮肤血流量呈高度线性;然而，当血流量低于基线时，相关性降低。这项研究的发现可能是在特定患者群体中使用LSCI的基础，例如烧伤护理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microcirculation 医学-外周血管病

CiteScore

5.00

自引率

4.20%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation. Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.