Expression of the Ovine Gene and the Relationship Between Its Polymorphism and Feed Efficiency Traits.

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

DNA and cell biology Pub Date : 2023-04-01 DOI:10.1089/dna.2022.0529

Xiwen Zeng, Weimin Wang, Deyin Zhang, Xiaolong Li, Yukun Zhang, Yuan Zhao, Liming Zhao, Jianghui Wang, Dan Xu, Jiangbo Cheng, Wenxin Li, Bubo Zhou, Changchun Lin, Xiaobin Yang, Rui Zhai, Zongwu Ma, Jia Liu, Panpan Cui, Xiuxiu Weng, Weiwei Wu, Xiaoxue Zhang, Wenxin Zheng

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引用次数: 1

Abstract

In the mutton industry, feed efficiency traits have the greatest influence on the economic benefits of sheep raised in housing conditions. In this study, quantitative real-time PCR (qRT-PCR), Sanger sequencing, and KASPar methods were used to detect the expression levels of the B cell scaffold protein with ankyrin repeats 1 (BANK1) gene and the relationship between its polymorphism and feed efficiency traits in Hu sheep. The qRT-PCR results showed that the BANK1 gene was extensively expressed in 10 tissues and it was expressed at remarkably higher levels in lymph than in other tissues (p < 0.05). Then, the polymorphism locus, g.93888 A > T, was detected in intron 4 of the BANK1 gene and proved to be remarkably associated with feed efficiency traits (p < 0.05). Hence, the BANK1 gene can be used as a candidate gene for improving the feed efficiency of Hu sheep and this locus could be used as a potential molecular marker for breeding high-feed efficiency sheep in future breeding efforts.

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绵羊基因的表达及其多态性与饲料效率性状的关系。

在肉羊行业中，饲料效率性状对圈养羊的经济效益影响最大。本研究采用实时荧光定量PCR (quantitative real-time PCR, qRT-PCR)、Sanger测序和KASPar方法检测湖羊B细胞支架蛋白锚蛋白重复序列1 (BANK1)基因的表达水平及其多态性与饲料效率性状的关系。qRT-PCR结果显示，BANK1基因在10个组织中广泛表达，且其在淋巴中的表达水平显著高于其他组织(p T;在BANK1基因的内含子4中检测到，与饲料效率性状显著相关(p BANK1基因可作为提高湖羊饲料效率的候选基因，在今后的育种工作中可作为培育高饲料效率羊的潜在分子标记。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

DNA and cell biology 生物-生化与分子生物学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

1.5 months

期刊介绍： DNA and Cell Biology delivers authoritative, peer-reviewed research on all aspects of molecular and cellular biology, with a unique focus on combining mechanistic and clinical studies to drive the field forward. DNA and Cell Biology coverage includes: Gene Structure, Function, and Regulation Gene regulation Molecular mechanisms of cell activation Mechanisms of transcriptional, translational, or epigenetic control of gene expression Molecular Medicine Molecular pathogenesis Genetic approaches to cancer and autoimmune diseases Translational studies in cell and molecular biology Cellular Organelles Autophagy Apoptosis P bodies Peroxisosomes Protein Biosynthesis and Degradation Regulation of protein synthesis Post-translational modifications Control of degradation Cell-Autonomous Inflammation and Host Cell Response to Infection Responses to cytokines and other physiological mediators Evasive pathways of pathogens.