Assessment of an e-nose performance for the detection of COVID-19 specific biomarkers.

IF 3.7 4区医学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of breath research Pub Date : 2023-02-16 DOI:10.1088/1752-7163/acb9b2

Christelle Ghazaly, Krystyna Biletska, Etienne A Thevenot, Philippe Devillier, Emmanuel Naline, Stanislas Grassin-Delyle, Emmanuel Scorsone

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Abstract

Early, rapid and non-invasive diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is needed for the prevention and control of coronavirus disease 2019 (COVID-19). COVID-19 mainly affects the respiratory tract and lungs. Therefore, analysis of exhaled breath could be an alternative scalable method for reliable SARS-CoV-2 screening. In the current study, an experimental protocol using an electronic-nose ('e-nose') for attempting to identify a specific respiratory imprint in COVID-19 patients was optimized. Thus the analytical performances of the Cyranose^®, a commercial e-nose device, were characterized under various controlled conditions. In addition, the effect of various experimental conditions on its sensor array response was assessed, including relative humidity, sampling time and flow rate, aiming to select the optimal parameters. A statistical data analysis was applied to e-nose sensor response using common statistical analysis algorithms in an attempt to demonstrate the possibility to detect the presence of low concentrations of spiked acetone and nonanal in the breath samples of a healthy volunteer. Cyranose^®reveals a possible detection of low concentrations of these two compounds, in particular of 25 ppm nonanal, a possible marker of SARS-CoV-2 in the breath.

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评估用于检测COVID-19特异性生物标志物的电子鼻性能

预防和控制2019冠状病毒病(COVID-19)需要对严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)感染进行早期、快速和无创诊断。COVID-19主要影响呼吸道和肺部。因此，呼气分析可能是可靠的SARS-CoV-2筛查的一种可扩展的替代方法。在目前的研究中，优化了一种使用电子鼻(“电子鼻”)试图识别COVID-19患者特定呼吸印记的实验方案。因此，商用电子鼻装置Cyranose®的分析性能在各种控制条件下进行了表征。此外，还评估了相对湿度、采样时间和流量等不同实验条件对其传感器阵列响应的影响，以选择最优参数。利用常见的统计分析算法对电子鼻传感器响应进行了统计数据分析，试图证明在健康志愿者的呼吸样本中检测到低浓度的加药丙酮和壬醛的可能性。Cyranose®显示可能检测到低浓度的这两种化合物，特别是25 ppm的壬醛，这可能是呼吸中SARS-CoV-2的标志。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of breath research BIOCHEMICAL RESEARCH METHODS-RESPIRATORY SYSTEM

CiteScore

7.60

自引率

21.10%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics. Typical areas of interest include: Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research. Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments. Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway. Cellular and molecular level in vitro studies. Clinical, pharmacological and forensic applications. Mathematical, statistical and graphical data interpretation.