Immunogenicity of the Non-toxic Form of Staphylococcus Aureus α-Hemolysin Using a Chimeric Fusion in Mice.

IF 1.1 4区医学 Q4 IMMUNOLOGY

Iranian Journal of Immunology Pub Date : 2023-03-14 DOI:10.22034/iji.2023.82351.1575

Liquan Yu, Jian Dai, Fan Ziyao, Liang Chen, Daolong Liu, Zhijun Wu, Hunan Sun, Chunyu Tong, Yongzhong Yu, Baifen Song, Jinzhu Ma, Yudong Cui

{"title":"Immunogenicity of the Non-toxic Form of Staphylococcus Aureus α-Hemolysin Using a Chimeric Fusion in Mice.","authors":"Liquan Yu, Jian Dai, Fan Ziyao, Liang Chen, Daolong Liu, Zhijun Wu, Hunan Sun, Chunyu Tong, Yongzhong Yu, Baifen Song, Jinzhu Ma, Yudong Cui","doi":"10.22034/iji.2023.82351.1575","DOIUrl":null,"url":null,"abstract":"Background: Staphylococcus aureus is an opportunistic pathogen responsible for various infections with diverse clinical presentation and severity. The α-hemolysin is a major virulence factor in the pathogenesis of S. aureus infections.Objective: To produce a chimeric fusion protein for hemolytic detection of the S. aureus isolates and as a component of a multi-antigen vaccine.Methods: The fused strategy employed a flexible linker to incorporate the possible B cell and T cell determinants into one chimera (HlaD). The humoral and cellular response to the HlaD in mice was assessed to reveal a non-significant difference compared with the full-length α-hemolysin mutant (Hla H35L).Results: The results of the protective effect, the mimetic lung cell injury, and bacterial clearness demonstrated that the mice vaccinated with the HlaD alleviated the severity of the infection of the S. aureus, and the HlaD could similarly function with Hla H35L.Conclusion: The chimeric fusion (HlaD) provided a diagnostic antigen for hemolysis of the S. aureus strains and a potential vaccine component.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Immunology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.22034/iji.2023.82351.1575","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Staphylococcus aureus is an opportunistic pathogen responsible for various infections with diverse clinical presentation and severity. The α-hemolysin is a major virulence factor in the pathogenesis of S. aureus infections.

Objective: To produce a chimeric fusion protein for hemolytic detection of the S. aureus isolates and as a component of a multi-antigen vaccine.

Methods: The fused strategy employed a flexible linker to incorporate the possible B cell and T cell determinants into one chimera (HlaD). The humoral and cellular response to the HlaD in mice was assessed to reveal a non-significant difference compared with the full-length α-hemolysin mutant (Hla H35L).

Results: The results of the protective effect, the mimetic lung cell injury, and bacterial clearness demonstrated that the mice vaccinated with the HlaD alleviated the severity of the infection of the S. aureus, and the HlaD could similarly function with Hla H35L.

Conclusion: The chimeric fusion (HlaD) provided a diagnostic antigen for hemolysis of the S. aureus strains and a potential vaccine component.

查看原文本刊更多论文

利用小鼠嵌合融合研究无毒型金黄色葡萄球菌α-溶血素的免疫原性

背景:金黄色葡萄球菌是一种机会致病菌，可引起各种临床表现和严重程度不同的感染。α-溶血素是金黄色葡萄球菌感染的主要毒力因子。目的:制备一种用于金黄色葡萄球菌分离株溶血检测的嵌合融合蛋白，并作为多抗原疫苗的组成部分。方法:采用柔性连接体将可能的B细胞和T细胞决定因子整合到一个嵌合体中。小鼠对HlaD的体液和细胞反应评估显示与全长α-溶血素突变体(Hla H35L)相比无显著差异。结果:保护作用、模拟肺细胞损伤和细菌清除率的结果表明，接种HlaD的小鼠减轻了金黄色葡萄球菌感染的严重程度，并且HlaD具有与Hla H35L相似的功能。结论:嵌合融合抗原(HlaD)可作为金黄色葡萄球菌溶血的诊断抗原和潜在的疫苗成分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Iranian Journal of Immunology Medicine-Immunology and Allergy

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Immunology (I.J.I) is an internationally disseminated peer-reviewed publication and publishes a broad range of experimental and theoretical studies concerned with all aspects of immunology.