Physiologic Effects of Housing Rats in Metabolic Cages.

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Marta Giral, Clara Armengol, Amadeu Gavaldà
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引用次数: 0

Abstract

Currently, metabolic cages (MC) are the only way to achieve serial sampling of urine and feces in rodents. However, the use of this caging creates a dramatic change from an animal's usual microenvironment. Here we sought to examine the effect of MC on physiologic parameters that are stress-responsive in rats. We surgically implanted 8 male Wistar rats (weight, 150 to 175 g) with telemetric transmitters and allowed them to recover for at least 2 wk. At the beginning of the study, the rats were moved to conventional open-top cages, and telemetry recording was initiated. After 24 h, the rats were moved to MC or to another conventional cage and the recording continued for another 24 h. Finally, the rats were returned to their home cages, and telemetry recording was performed for a final 24 h. After 10 days, this process was then repeated, with MC and conventional assignments switched. During the 78-h monitoring period, we recorded heart rate, arterial blood pressure, locomotor activity, body weight, and food and water consumption. Heart rate and arterial blood pressure showed transient but significant changes. Locomotor activity during the dark phase was greatly decreased in MC compared with conventional cages, perhaps due to space constraints. In addition, when the rats were housed in MC, they showed a small but significant weight loss. Food consumption did not differ between housing environments, but water consumption was lower when rats were in MC. In conclusion, the housing of rats in MC for 24 h can elicit mild and reversible cardiovascular changes. This finding is consistent with European Directive 2010/63/EU, which considers short-term (less than 24 h) restraint in MC a procedure of mild severity.

代谢笼饲养大鼠的生理效应。
目前,代谢笼(MC)是实现啮齿动物尿液和粪便连续采样的唯一方法。然而,这种笼子的使用与动物通常的微环境产生了巨大的变化。在这里,我们试图检查MC对大鼠应激反应生理参数的影响。我们将8只体重150 ~ 175 g的雄性Wistar大鼠手术植入遥测发射机,并让它们恢复至少2周。在研究开始时,将大鼠转移到传统的开顶笼中,并开始遥测记录。24小时后,将大鼠转移到MC或另一个常规笼子,继续记录24小时。最后,将大鼠送回其家庭笼子,并进行最后24小时的遥测记录。10天后,重复这一过程,MC和常规任务互换。在78小时的监测期间,我们记录了心率、动脉血压、运动活动、体重、食物和水的消耗。心率和动脉血压出现短暂但显著的变化。与传统笼相比,MC在暗期的运动活动大大减少,可能是由于空间限制。此外,当大鼠被安置在MC中时,它们表现出轻微但显著的体重减轻。在不同的饲养环境下,大鼠的食物消耗没有差异,但水消耗较低。综上所述,大鼠在MC中饲养24 h可引起轻度和可逆的心血管变化。这一发现与欧洲指令2010/63/EU一致,该指令认为短期(少于24小时)MC约束是轻度严重程度的程序。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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