Investigation into the effect of systemic single high-dose erythropoietin on the healing of Achilles tendons in rats.

IF 1.1 4区医学 Q3 ORTHOPEDICS

Acta orthopaedica et traumatologica turcica Pub Date : 2022-11-01 DOI:10.5152/j.aott.2022.22106

Yasin Köker, Mehmet Armangil, Mert Karaduman, Gözde Yücel Tenekeci, Baver Acar, Burak Akan

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Abstract

Objective: This study aimed to examine systemic erythropoietin's effect on the Achilles tendon's healing in a rat model.

Methods: Twenty-five adult Wistar rats were randomly assigned to one of two groups. The Achilles tendon of each rat was transected 5 mm proximal to its insertion to the calcaneus. All Achilles tendons were then repaired using modified Kessler methods. A single dose (5000 U/kg) of intraperitoneal erythropoietin (EPO) was administered to group I. Group II was a control group and did not receive an EPO injection. Four rats from each group were sacrificed at 1, 3 and 6 weeks after injection. Histopathological assessments were performed by observers blinded to the treatment.

Results: Groups I and II showed a similar increase in fibroblast cytoplasmic content and fibrillar collagen in the extracellular matrix. Collagen deposition, cellular proliferation, number of lipid vacuoles and capillary increases were similar between the groups.

Conclusion: Evidence from this study has shown no direct effect of a single systemic high dose of EPO on the histological properties of the Achilles tendon in rats.

Abstract Image

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全身单次大剂量促红细胞生成素对大鼠跟腱愈合影响的研究。

目的:探讨全身促红细胞生成素对大鼠跟腱愈合的影响。方法:25只成年Wistar大鼠随机分为两组。取每只大鼠跟腱距跟骨止点近5mm处横切。然后使用改良的Kessler方法修复所有跟腱。ⅰ组腹腔注射单剂量(5000 U/kg)促红细胞生成素(EPO)，ⅱ组为对照组，未注射促红细胞生成素。各组分别于注射后1、3、6周处死4只大鼠。组织病理学评估由对治疗不知情的观察者进行。结果:ⅰ组和ⅱ组成纤维细胞细胞质含量和细胞外基质中纤维性胶原蛋白均有相似的增加。各组间胶原沉积、细胞增殖、脂泡数量及毛细血管增加无明显差异。结论:本研究的证据表明，单次全身大剂量EPO对大鼠跟腱的组织学特性没有直接影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta orthopaedica et traumatologica turcica ORTHOPEDICS-

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Acta Orthopaedica et Traumatologica Turcica (AOTT) is an international, scientific, open access periodical published in accordance with independent, unbiased, and double-blinded peer-review principles. The journal is the official publication of the Turkish Association of Orthopaedics and Traumatology, and Turkish Society of Orthopaedics and Traumatology. It is published bimonthly in January, March, May, July, September, and November. The publication language of the journal is English. The aim of the journal is to publish original studies of the highest scientific and clinical value in orthopedics, traumatology, and related disciplines. The scope of the journal includes but not limited to diagnostic, treatment, and prevention methods related to orthopedics and traumatology. Acta Orthopaedica et Traumatologica Turcica publishes clinical and basic research articles, case reports, personal clinical and technical notes, systematic reviews and meta-analyses and letters to the Editor. Proceedings of scientific meetings are also considered for publication. The target audience of the journal includes healthcare professionals, physicians, and researchers who are interested or working in orthopedics and traumatology field, and related disciplines.