Mice lacking proSAAS display alterations in emotion, consummatory behavior and circadian entrainment

IF 2.4 4区 心理学 Q2 BEHAVIORAL SCIENCES
Dipendra K. Aryal, Ramona M. Rodriguiz, Ngoc Lien Nguyen, Matthew W. Pease, Daniel J. Morgan, John Pintar, Lloyd D. Fricker, William C. Wetsel
{"title":"Mice lacking proSAAS display alterations in emotion, consummatory behavior and circadian entrainment","authors":"Dipendra K. Aryal,&nbsp;Ramona M. Rodriguiz,&nbsp;Ngoc Lien Nguyen,&nbsp;Matthew W. Pease,&nbsp;Daniel J. Morgan,&nbsp;John Pintar,&nbsp;Lloyd D. Fricker,&nbsp;William C. Wetsel","doi":"10.1111/gbb.12827","DOIUrl":null,"url":null,"abstract":"<p>ProSAAS is a neuroendocrine protein that is cleaved by neuropeptide-processing enzymes into more than a dozen products including the bigLEN and PEN peptides, which bind and activate the receptors GPR171 and GPR83, respectively. Previous studies have suggested that proSAAS-derived peptides are involved in physiological functions that include body weight regulation, circadian rhythms and anxiety-like behavior. In the present study, we find that proSAAS knockout mice display robust anxiety-like behaviors in the open field, light–dark emergence and elevated zero maze tests. These mutant mice also show a reduction in cued fear and an impairment in fear-potentiated startle, indicating an important role for proSAAS-derived peptides in emotional behaviors. ProSAAS knockout mice exhibit reduced water consumption and urine production relative to wild-type controls. No differences in food consumption and overall energy expenditure were observed between the genotypes. However, the respiratory exchange ratio was elevated in the mutants during the light portion of the light–dark cycle, indicating decreased fat metabolism during this period. While proSAAS knockout mice show normal circadian patterns of activity, even upon long-term exposure to constant darkness, they were unable to shift their circadian clock upon exposure to a light pulse. Taken together, these results show that proSAAS-derived peptides modulate a wide range of behaviors including emotion, metabolism and the regulation of the circadian clock.</p>","PeriodicalId":50426,"journal":{"name":"Genes Brain and Behavior","volume":"21 7","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/78/fc/GBB-21-e12827.PMC9444949.pdf","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genes Brain and Behavior","FirstCategoryId":"102","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/gbb.12827","RegionNum":4,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BEHAVIORAL SCIENCES","Score":null,"Total":0}
引用次数: 2

Abstract

ProSAAS is a neuroendocrine protein that is cleaved by neuropeptide-processing enzymes into more than a dozen products including the bigLEN and PEN peptides, which bind and activate the receptors GPR171 and GPR83, respectively. Previous studies have suggested that proSAAS-derived peptides are involved in physiological functions that include body weight regulation, circadian rhythms and anxiety-like behavior. In the present study, we find that proSAAS knockout mice display robust anxiety-like behaviors in the open field, light–dark emergence and elevated zero maze tests. These mutant mice also show a reduction in cued fear and an impairment in fear-potentiated startle, indicating an important role for proSAAS-derived peptides in emotional behaviors. ProSAAS knockout mice exhibit reduced water consumption and urine production relative to wild-type controls. No differences in food consumption and overall energy expenditure were observed between the genotypes. However, the respiratory exchange ratio was elevated in the mutants during the light portion of the light–dark cycle, indicating decreased fat metabolism during this period. While proSAAS knockout mice show normal circadian patterns of activity, even upon long-term exposure to constant darkness, they were unable to shift their circadian clock upon exposure to a light pulse. Taken together, these results show that proSAAS-derived peptides modulate a wide range of behaviors including emotion, metabolism and the regulation of the circadian clock.

Abstract Image

缺乏proSAAS的小鼠在情绪、完成行为和昼夜节律方面表现出改变
ProSAAS是一种神经内分泌蛋白,可被神经肽加工酶裂解成十几种产物,包括bigLEN和PEN肽,它们分别结合并激活受体GPR171和GPR83。先前的研究表明,prosaas衍生的肽参与了包括体重调节、昼夜节律和焦虑样行为在内的生理功能。在本研究中,我们发现proSAAS基因敲除小鼠在开阔场地、明暗突现和升高零迷宫测试中表现出稳健的焦虑样行为。这些突变小鼠还显示出暗示恐惧的减少和恐惧增强惊吓的损伤,表明prosaas衍生肽在情绪行为中起重要作用。与野生型对照相比,ProSAAS基因敲除小鼠表现出更少的水消耗和尿产生。基因型之间的食物消耗和总能量消耗没有差异。然而,突变体的呼吸交换比率在光-暗循环的光部分升高,表明在此期间脂肪代谢降低。尽管proSAAS基因敲除小鼠表现出正常的昼夜节律活动模式,即使长期暴露在持续的黑暗中,它们也无法在暴露于光脉冲时改变昼夜节律钟。综上所述,这些结果表明prosaas衍生的肽调节了广泛的行为,包括情绪、代谢和生物钟的调节。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Genes Brain and Behavior
Genes Brain and Behavior 医学-行为科学
CiteScore
6.80
自引率
4.00%
发文量
62
审稿时长
4-8 weeks
期刊介绍: Genes, Brain and Behavior was launched in 2002 with the aim of publishing top quality research in behavioral and neural genetics in their broadest sense. The emphasis is on the analysis of the behavioral and neural phenotypes under consideration, the unifying theme being the genetic approach as a tool to increase our understanding of these phenotypes. Genes Brain and Behavior is pleased to offer the following features: 8 issues per year online submissions with first editorial decisions within 3-4 weeks and fast publication at Wiley-Blackwells High visibility through its coverage by PubMed/Medline, Current Contents and other major abstracting and indexing services Inclusion in the Wiley-Blackwell consortial license, extending readership to thousands of international libraries and institutions A large and varied editorial board comprising of international specialists.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信