Effects of Transdermal Fentanyl Treatment on Acute Pain and Inflammation in Rats with Adjuvant-induced Monoarthritis.

IF 1.3 4区农林科学 Q2 VETERINARY SCIENCES

Comparative medicine Pub Date : 2022-10-01 DOI:10.30802/AALAS-CM-21-000066

Mie S Berke, Pernille Colding-Jørgensen, Line G Pedersen, Sara Hestehave, Otto Kalliokoski, Henrik E Jensen, Dorte B Sørensen, Jann Hau, Klas Sp Abelson

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引用次数: 1

Abstract

Eliminating unnecessary pain is an important requirement of performing animal experimentation, including reducing and controlling pain of animals used in pain research. The goal of this study was to refine an adjuvant-induced monoarthritis model in rats by providing analgesia with a transdermal fentanyl solution (TFS). Male and female Sprague-Dawley rats, single- or pair-housed, were injected with 20 μL of complete Freund adjuvant (CFA) into the left ankle joint. CFA-injected rats treated with a single dose of transdermal fentanyl solution (0.33 or 1 mg/kg) were compared with an untreated CFA-injected group and sham groups that received either no treatment or TFS treatment (1 mg/kg) during 72 h. At the tested doses, TFS reduced mechanical hyperalgesia and improved the mobility, stance, rearing, and lameness scores at 6 h after CFA injection. Joint circumferences were not reduced by TFS treatment, and no significant differences were detected between the 2 doses of TFS, or between single- and pair-housed rats. Treatment with TFS did not appear to interfere with model development and characteristics. However, overall, the analgesic effect was transient, and several opioid-related side effects were observed.

查看原文本刊更多论文

芬太尼经皮治疗对佐剂性单关节炎大鼠急性疼痛和炎症的影响。

消除不必要的疼痛是进行动物实验的重要要求，包括减少和控制用于疼痛研究的动物的疼痛。本研究的目的是通过给芬太尼透皮溶液(TFS)提供镇痛来完善大鼠佐剂诱导的单关节炎模型。将雄性和雌性Sprague-Dawley大鼠单独或成对饲养，在左踝关节内注射20 μL完全弗氏佐剂(CFA)。在72小时内，将单剂量芬太尼透皮溶液(0.33或1 mg/kg)治疗的CFA注射大鼠与未治疗的CFA注射组和未接受治疗或TFS治疗(1 mg/kg)的假手术组进行比较。在测试剂量下，TFS减少了机械性痛觉过敏，改善了CFA注射后6小时的活动能力、站立能力、饲养能力和瘸腿评分。TFS治疗没有减少关节周长，并且在两种剂量的TFS之间，或者在单和成对饲养的大鼠之间没有发现显著差异。用TFS治疗似乎不会干扰模型的发展和特征。然而，总的来说，镇痛作用是短暂的，并且观察到一些阿片类药物相关的副作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Comparative medicine 医学-动物学

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.