Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute Leukemia.

IF 1.1 4区医学 Q4 IMMUNOLOGY

Iranian Journal of Immunology Pub Date : 2023-12-31 Epub Date: 2023-11-14 DOI:10.22034/iji.2023.97902.2537

Maryam Mohammadi, Hossein Asgarian-Omran, Behnam Najafi, Ahmad Najafi, Reza Valadan, Hossein Karami, Mohammad Naderisoraki, Maryam Alizadeforoutan, Ramin Shekarriz, Mohsen Tehrani

{"title":"Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute Leukemia.","authors":"Maryam Mohammadi, Hossein Asgarian-Omran, Behnam Najafi, Ahmad Najafi, Reza Valadan, Hossein Karami, Mohammad Naderisoraki, Maryam Alizadeforoutan, Ramin Shekarriz, Mohsen Tehrani","doi":"10.22034/iji.2023.97902.2537","DOIUrl":null,"url":null,"abstract":"Background: Thymocyte selection-associated high mobility group box protein (TOX) and members of the nuclear receptor 4A (NR4A) are known as transcription factors involved in T cell exhaustion.Objective: To evaluate the mRNA expression of TOX and NR4A1-3 in CD8+ T cells in acute leukemia.Methods: Blood samples were obtained from 21 ALL and 6 AML patients as well as 20 control subjects. CD8+ T cells were isolated using MACS. Relative gene expression of TOX and NR4A1-3 was then evaluated using qRT-PCR.Results: Comparison of mRNA expression of TOX in CD8+ T cells showed no significant difference among the study groups (p>0.05), while the expression of NR4A1 was significantly lower in AML patients than in the control group (p=0.0006). Also, the expression of NR4A2 and NR4A3 was significantly lower in both ALL (p=0.0049 and p=0.0005, respectively) and AML (p=0.0019 and p=0.0055, respectively) patients.Conclusion: NR4As expressions were found to be lower in CD8+ T cells from patients with AML and ALL compared to controls, whereas the mRNA expression of TOX showed no significant difference. Although TOX and NR4As are associated with CD8+ T cell exhaustion in solid tumors, they might play different roles in acute leukemia, which requires further investigation.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Immunology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.22034/iji.2023.97902.2537","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/11/14 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Thymocyte selection-associated high mobility group box protein (TOX) and members of the nuclear receptor 4A (NR4A) are known as transcription factors involved in T cell exhaustion.

Objective: To evaluate the mRNA expression of TOX and NR4A1-3 in CD8+ T cells in acute leukemia.

Methods: Blood samples were obtained from 21 ALL and 6 AML patients as well as 20 control subjects. CD8+ T cells were isolated using MACS. Relative gene expression of TOX and NR4A1-3 was then evaluated using qRT-PCR.

Results: Comparison of mRNA expression of TOX in CD8+ T cells showed no significant difference among the study groups (p>0.05), while the expression of NR4A1 was significantly lower in AML patients than in the control group (p=0.0006). Also, the expression of NR4A2 and NR4A3 was significantly lower in both ALL (p=0.0049 and p=0.0005, respectively) and AML (p=0.0019 and p=0.0055, respectively) patients.

Conclusion: NR4As expressions were found to be lower in CD8+ T cells from patients with AML and ALL compared to controls, whereas the mRNA expression of TOX showed no significant difference. Although TOX and NR4As are associated with CD8+ T cell exhaustion in solid tumors, they might play different roles in acute leukemia, which requires further investigation.

查看原文本刊更多论文

急性白血病CD8+ T细胞TOX和NR4As mRNA表达的评价。

背景:胸腺细胞选择相关的高迁移率组盒蛋白(TOX)和核受体4A (NR4A)成员是参与T细胞衰竭的转录因子。目的:探讨急性白血病CD8+ T细胞中TOX和NR4A1-3 mRNA的表达。方法:采集急性淋巴细胞白血病患者21例、急性髓系白血病患者6例及对照组20例。采用MACS法分离CD8+ T细胞。采用qRT-PCR方法检测TOX和NR4A1-3基因的相对表达量。结果:两组间CD8+ T细胞中TOX mRNA表达比较差异无统计学意义(p < 0.05)，而AML患者中NR4A1 mRNA表达明显低于对照组(p < 0.0006)。此外，NR4A2和NR4A3在ALL (p=0.0049和p=0.0005)和AML (p=0.0019和p=0.0055)患者中的表达均显著降低。结论:AML和ALL患者CD8+ T细胞中NR4As表达低于对照组，而TOX mRNA表达差异无统计学意义。虽然TOX和NR4As在实体肿瘤中与CD8+ T细胞衰竭相关，但它们在急性白血病中可能发挥不同的作用，有待进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Iranian Journal of Immunology Medicine-Immunology and Allergy

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Immunology (I.J.I) is an internationally disseminated peer-reviewed publication and publishes a broad range of experimental and theoretical studies concerned with all aspects of immunology.