The Aα Mating-Type Transcripts of Schizophyllum commune

Huiling Yang, Guang-Ping Shen, Dong Chul Park, Charles P. Novotny, Robert C. Ullrich
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引用次数: 9

Abstract

Yang, H., Shen, G-P., Park, D.C., Novotny, C. P., and Ullrich, R. C. 1995. The Aα mating-type transcripts of Schizophyllum commune. Experimental Mycology 19, 16-25. Aα1, Aα3, and Aα4 ds- and ss-DNA probes from the polymorphic Aα mating-type locus of Schizophyllum commune were used to probe Northern blots of poly(A+) RNA extracted from strains of various Aα mating types. The purpose of these experiments was to identify, map, and characterize the transcripts produced from the regions of the Aα locus. The transcripts unique to Aα mating type map colinear with the open reading frames identified from DNA sequence and are encoded within the fragments which activate the A developmental pathway in transformation. These data confirm the existence and structure of the previously hypothesized Y and Z Aα mating-type genes. Transcripts from the Y and Z genes are present in vegetative cells of homokaryons and dikaryons and in cells of the fruiting bodies. The presence of the transcripts throughout the life cycle is consistent with the model of Y and Z proteins as "master switches" of A-regulated development.

裂叶属植物Aα交配型转录本的研究
杨辉,沈国平。, Park, d.c., Novotny, c.p.和Ullrich, r.c. 1995。裂叶植物群落的Aα交配型转录本。实验真菌学19,16-25。利用裂叶菌(Schizophyllum commune)多态Aα交配型位点的Aα1、Aα3和Aα4 ds-和ss-DNA探针,对从不同Aα交配型菌株中提取的poly(A+) RNA进行Northern印迹检测。这些实验的目的是鉴定、定位和表征从Aα位点区域产生的转录本。Aα交配型特有的转录本与从DNA序列中鉴定出的开放阅读框共线,并编码在转化中激活A发育途径的片段内。这些数据证实了先前假设的Y和Z Aα交配型基因的存在和结构。Y和Z基因的转录本存在于同核体和双核体的营养细胞以及子实体的细胞中。转录本在整个生命周期中的存在与Y和Z蛋白作为a调控发育的“主开关”的模型是一致的。
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