Estresse oxidativo sistêmico e folicular em mulheres inférteis com endometriose submetidas à injeção intracitoplasmática de espermatozoide

Reprodu??o & Climatério Pub Date : 2014-09-01 DOI:10.1016/j.recli.2015.04.005

Michele Gomes Da Broi , Felipe Oliveira de Albuquerque , Aline Zyman de Andrade , Alceu Afonso Jordão Junior , Paula Andrea Navarro

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引用次数: 1

Abstract

Objectives

The aim of the study was to compare eight oxidative stress (OS) markers in serum and follicular fluid (FF) of infertile women with and without endometriosis (E) undergoing controlled ovarian stimulation (COS) for intracytoplasmic sperm injection (ICSI).

Material and methods

Eight‐seven serum samples (43 with E and 44 without E ‐ with male or tubal factor) and 61 FF samples (29 with E and 32 without E) were collected in the day of oocyte retrieval. Total hydroperoxides (FOX₁), malondialdehyde (MDA), advanced oxidation protein products (AOPP), glutathione (GSH), superoxide dismutase (SOD) and the total antioxidant capacity (TAC) were determined by spectrophotometry, vitamin E (Vit E) by high performance liquid chromatography, and 8‐hydroxy‐2’–deoxyguanosine (8 OHdG) by ELISA.

Results

We observed higher serum concentrations of GSH (220.32 ± 43.2 nmol/g pt) and SOD (677.9 ± 282.21 U/mL), lower serum concentrations of TAC (0.34 ± 0.17 mEq Trolox/L), and higher follicular concentrations of 8 OHdG (23.19 ± 6.8 ng/mL) and Vit E (13.0 ± 5.33 μmol/L) in infertile women with E compared to those without E (193.92 ± 43.25 nmol/g pt, 563.04 ± 169.82 U/mL, 0.46 ± 0.15 mEq Trolox/L, 17.22 ± 5.6 ng/mL and 8.71 ± 2.51 μmol/L, respectively).

Conclusions

We evidenced the occurrence of systemic and follicular OS in infertile patients with E undergoing COS for ICSI. We suggest that the OS may be involved in the etiopathogenesis of infertility‐related disease

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子宫内膜异位症不孕妇女胞浆内精子注射的全身和卵泡氧化应激

目的比较有和无子宫内膜异位症(E)的不孕妇女在接受控制卵巢刺激(COS)进行胞浆内单精子注射(ICSI)时血清和卵泡液(FF)中8种氧化应激(OS)标志物的变化。材料与方法取卵当天共收集8 - 7份血清样本(43份含E, 44份不含E)和61份FF样本(29份含E, 32份不含E)。分光光度法测定总氢过氧化物(FOX1)、丙二醛(MDA)、高级氧化蛋白产物(AOPP)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)和总抗氧化能力(TAC)，高效液相色谱法测定维生素E (Vit E)， ELISA法测定8‑羟基‑2′‑脱氧鸟苷(8‑OHdG)。ResultsWe观察高血清浓度的谷胱甘肽(220.32±43.2 nmol / g pt)和SOD(677.9±282.21 U /毫升),低血清浓度的TAC(0.34±0.17毫克当量Trolox / L),和更高的卵泡8 OHdG浓度(23.19±6.8 ng / mL)和E(13.0±5.33μmol / L)与E不孕妇女相比,那些没有E(193.92±43.25 nmol / g pt, 563.04±169.82 U / mL, 0.46±0.15毫克当量Trolox / L, 17.22±5.6 ng / mL, 8.71±2.51μmol / L,分别)。结论我们证实了在输卵管内精子注射(ICSI)的不育患者中有系统性和卵泡性OS的发生。我们认为OS可能参与了不孕相关疾病的发病机制

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Reprodu??o & Climatério

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