Small RNA-seq analysis in response to methyl jasmonate and abscisic acid treatment in Persicaria minor

Nazaruddin Nazaruddin , Abdul Fatah A. Samad , Muhammad Sajad , Jaeyres Jani , Zamri Zainal , Ismanizan Ismail
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引用次数: 4

Abstract

Persicaria minor (Kesum) is an important medicinal plant with high level of secondary metabolite contents, especially, terpenoids and flavonoids. Previous studies have revealed that application of exogenous phytohormone could increase secondary metabolite contents of the plant. MicroRNAs (miRNAs) are small RNAs that play important regulatory roles in various biological processes. In order to explore the possible role of miRNA in the regulation of these phytohormones signaling pathway and uncovering their potential correlation, we, for the first time, have generated the smallRNA library of Kesum plant. The library was developed in response to methyl jasmonate (MJ) and abscisic acid (ABA) treatment by using next-generation sequencing technology. Raw reads have been deposited to SRA database with the accession numbers, SRX2655642 and SRX2655643 (MJ-treated), SRXSRX2655644 and SRX2655645 (ABA-treated) and SRX2655646and SRX2655647 (Control).

小仙桃对茉莉酸甲酯和脱落酸处理的小RNA-seq分析
小木桃(Persicaria minor, Kesum)是一种重要的药用植物,其次生代谢产物尤其是萜类和黄酮类物质含量较高。以往的研究表明,外源植物激素的施用可以增加植物次生代谢物的含量。MicroRNAs (miRNAs)是在多种生物过程中发挥重要调控作用的小rna。为了探索miRNA在这些植物激素信号通路调控中的可能作用,揭示它们之间的潜在关联,我们首次构建了Kesum植物的smallRNA文库。利用新一代测序技术对茉莉酸甲酯(methyl jasmonate, MJ)和脱落酸(abscisic acid, ABA)进行处理,建立了该文库。原始读取数据已存入SRA数据库,登记号为SRX2655642和SRX2655643 (mj处理),SRXSRX2655644和SRX2655645 (aba处理),srx2655646和SRX2655647 (Control)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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