Oviductal sperm storage in the Chinese pond turtle, Mauremys reevesii, depends on androgen-based promotion of the BCL 2 anti-apoptotic pathway.

IF 1.8 4区生物学 Q3 DEVELOPMENTAL BIOLOGY

Reproduction, fertility, and development Pub Date : 2023-03-01 DOI:10.1071/RD22205

Wei Chen, Yajie Wang, Mengmeng Gong, Hailin Gao, Huaxing Zhou, Xingjiang Bu, Xingquan Xia, Liuwang Nie

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引用次数: 0

Abstract

Context: Sperm storage is a complex and highly coordinated process that is regulated by a variety of factors. The BCL 2 protein family plays a key role in regulating apoptosis, and determines sperm survival.

Aims: The objective of this study was to explore the correlation between sperm storage and the BCL 2 protein family in the oviduct of Mauremys reevesii .

Methods: Hematoxylin-eosin (HE) staining, immunohistochemistry (IHC) and real-time quantitative polymerase chain reaction (RT-qPCR) techniques were used to investigate three parts of the reproductive tract (isthmus, uterus and vagina) of mated and unmated female M. reevesii .

Key results: Hematoxylin-eosin staining revealed many sperm stored in the oviduct. IHC showed positive immunostaining for the BCL 2 and BAX proteins in epithelial ciliated and glandular cells. RT-qPCR indicated that the mRNA expressions of anti-apoptotic genes (BCL 2 , MCL 1 , BCL- W , BCL-XL ) and the androgen receptor (AR) were significantly higher in mated turtles than unmated turtles. However, the expression of pro-apoptotic genes (BAX , BAD , BID and CASPASE 3 ) showed the opposite relationship.

Conclusions: These results suggest that sperm entering the oviduct can promote the synthesis of anti-apoptotic genes to protect themselves from various degradation factors.

Implications: These findings will help researchers understand the mechanisms of sperm storage.

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中华塘龟(maremys reevesii)输卵管精子储存依赖于基于雄激素的BCL 2抗凋亡通路的促进。

背景:精子储存是一个复杂而高度协调的过程，受多种因素的调节。BCL - 2蛋白家族在调节细胞凋亡和决定精子存活中起关键作用。目的:探讨精子储存与生殖道BCL - 2蛋白家族的关系。方法:采用苏木精伊红(HE)染色、免疫组化(IHC)和实时定量聚合酶链反应(RT-qPCR)技术，对交配和未交配雌性生殖道峡部、子宫和阴道三个部位进行观察。苏木精-伊红染色显示输卵管内储存有许多精子。免疫组化染色显示上皮纤毛细胞和腺细胞BCL 2和BAX蛋白阳性。RT-qPCR结果显示，偶鳖抗凋亡基因(BCL 2、mccl 1、BCL- W、BCL- xl)和雄激素受体(AR) mRNA表达量显著高于未偶鳖。而促凋亡基因(BAX、BAD、BID、CASPASE 3)的表达则呈相反的关系。结论:精子进入输卵管后可促进抗凋亡基因的合成，保护自身免受各种降解因素的影响。启示:这些发现将有助于研究人员了解精子储存的机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproduction, fertility, and development 生物-动物学

CiteScore

2.10

自引率

10.50%

发文量

317

审稿时长

2 months

期刊介绍： Reproduction, Fertility and Development is an international journal for the publication of original and significant contributions on vertebrate reproductive and developmental biology. Subject areas include, but are not limited to: physiology, biochemistry, cell and molecular biology, endocrinology, genetics and epigenetics, behaviour, immunology and the development of reproductive technologies in humans, livestock and wildlife, and in pest management. Reproduction, Fertility and Development is a valuable resource for research scientists working in industry or academia on reproductive and developmental biology, clinicians and veterinarians interested in the basic science underlying their disciplines, and students. Reproduction, Fertility and Development is the official journal of the International Embryo Technology Society and the Society for Reproductive Biology. Reproduction, Fertility and Development is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.