Diagnostics and Therapeutic Potential of miR-205 and miR-34a in Ovarian Cancer Management: A miRNA-Target-Based Analysis.

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

DNA and cell biology Pub Date : 2023-03-01 DOI:10.1089/dna.2022.0487

Vivek Kumar, Archana Pandey, Arisha Arora, Priyanka Gautam, Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan

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引用次数: 0

Abstract

Epithelial ovarian cancer (EOC) treatment strategies mainly focused on surgery combined with chemotherapy. Recent targeted therapy techniques emerge as milestone and could be used for management of ovarian cancer (OC) progression with more efficacy. The aim is to evaluate the therapeutic and diagnostic potential of microRNA (miRNA) in management of EOC using in silico and quantitative real-time PCR (qRT-PCR) expression analysis. We performed functional enrichment and miRNA-Target genes expression analysis in 48 EOC and 22 normal tissue samples using qRT-PCR and correlated with miRNA expression data in matched samples to evaluate the diagnostic and therapeutic potential of miRNA in OC management. In silico functional enrichment analysis revealed miRNA association with disease. Target genes of miRNAs participate in several biologically important pathways leading to cancer progression. Targets of miRNA-205 and miRNA-34a were significantly downregulated, and upregulated, respectively, in EOC. Moreover, significant negative correlation between relative expression of miRNA-205 and target genes (BCL2, ZEB1, E2F1, and TP53) was observed with r = -0.813; r = -0.755; r = -0.559; and r = -0.767, respectively. Similarly, miRNA-34a also showed higher negative correlation with target genes (MDM4, MAPK3, BRCA1, AREG) with r = -0.840; r = -0.870; r = -0.622; and r = -0.623, respectively. In addition, receiver operating characteristics analysis of combined miRNA panel, miRNA-205-Target gene panel, and miRNA-34a-Target gene panel exhibited higher diagnostics value with area under the curve (AUC) of 92.7 (p < 0.0001), 94.8 (p < 0.0001), and 98.3 (p < 0.0001), respectively. Negative Correlation between miRNA and target genes expression data in matched samples highlights therapeutic potential of miRNA in EOC management. Moreover, combined diagnostic potential of miRNA-target gene panel could predict risk of EOC with higher AUC, sensitivity, and specificity.

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miR-205和miR-34a在卵巢癌治疗中的诊断和治疗潜力:基于mirna靶点的分析

上皮性卵巢癌(EOC)的治疗策略主要以手术联合化疗为主。最近的靶向治疗技术出现了一个里程碑，可以更有效地用于卵巢癌(OC)进展的管理。目的是利用计算机和定量实时PCR (qRT-PCR)表达分析来评估microRNA (miRNA)在EOC治疗和诊断中的潜力。我们使用qRT-PCR对48例EOC和22例正常组织样本进行了功能富集和miRNA- target基因表达分析，并与匹配样本中的miRNA表达数据进行了关联，以评估miRNA在OC治疗中的诊断和治疗潜力。功能富集分析显示miRNA与疾病相关。mirna的靶基因参与了导致癌症进展的几种重要生物学途径。在EOC中，miRNA-205和miRNA-34a的靶点分别显著下调和上调。miRNA-205与靶基因BCL2、ZEB1、E2F1、TP53的相对表达量呈显著负相关(r = -0.813);r = -0.755;r = -0.559;r = -0.767。同样，miRNA-34a也与靶基因MDM4、MAPK3、BRCA1、AREG呈较高的负相关，r = -0.840;r = -0.870;r = -0.622;和r = -0.623。此外，联合miRNA面板、miRNA-205- target基因面板和miRNA-34a- target基因面板的受试者工作特征分析显示出更高的诊断价值，曲线下面积(AUC)为92.7 (p p p)

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来源期刊

DNA and cell biology 生物-生化与分子生物学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

1.5 months

期刊介绍： DNA and Cell Biology delivers authoritative, peer-reviewed research on all aspects of molecular and cellular biology, with a unique focus on combining mechanistic and clinical studies to drive the field forward. DNA and Cell Biology coverage includes: Gene Structure, Function, and Regulation Gene regulation Molecular mechanisms of cell activation Mechanisms of transcriptional, translational, or epigenetic control of gene expression Molecular Medicine Molecular pathogenesis Genetic approaches to cancer and autoimmune diseases Translational studies in cell and molecular biology Cellular Organelles Autophagy Apoptosis P bodies Peroxisosomes Protein Biosynthesis and Degradation Regulation of protein synthesis Post-translational modifications Control of degradation Cell-Autonomous Inflammation and Host Cell Response to Infection Responses to cytokines and other physiological mediators Evasive pathways of pathogens.