{"title":"Properties of a biosurfactant produced by the fuel contaminant Ochrobactrum anthropii","authors":"M.P. Wasko, R.P. Bratt","doi":"10.1016/0265-3036(91)90055-V","DOIUrl":null,"url":null,"abstract":"<div><p>A bacterium <em>Ochrobactrum anthropii</em> isolated from contaminated fuel had a strong ability, associated with a cell-bound component, to emulsify hydrocarbon: water mixtures. This was only released into the medium following extraction involving sonication and ultracentrifugation. The component was partially purified using a fast protein liquid chromatography system. From this and polyacrylamide gel electrophoresis, it was found to be essentially a protein of molecular weight 1·04 × 10<sup>5</sup>. Enzyme digests of the crude extract prepared using protease revealed that a protein component was essential for activity. The emulsifying ability of the crude extract was assessed against a variety of hydrocarbon: water systems utilising aliphatic, aromatic and cyclic hydrocarbon. A broad range of hydrocarbons was emulsified.</p></div>","PeriodicalId":13629,"journal":{"name":"International Biodeterioration","volume":"27 3","pages":"Pages 265-273"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0265-3036(91)90055-V","citationCount":"21","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Biodeterioration","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/026530369190055V","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 21
Abstract
A bacterium Ochrobactrum anthropii isolated from contaminated fuel had a strong ability, associated with a cell-bound component, to emulsify hydrocarbon: water mixtures. This was only released into the medium following extraction involving sonication and ultracentrifugation. The component was partially purified using a fast protein liquid chromatography system. From this and polyacrylamide gel electrophoresis, it was found to be essentially a protein of molecular weight 1·04 × 105. Enzyme digests of the crude extract prepared using protease revealed that a protein component was essential for activity. The emulsifying ability of the crude extract was assessed against a variety of hydrocarbon: water systems utilising aliphatic, aromatic and cyclic hydrocarbon. A broad range of hydrocarbons was emulsified.
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