Variability of the prion protein gene (PRNP) in Algerian dromedary populations

Q4 Biochemistry, Genetics and Molecular Biology

Animal Gene Pub Date : 2020-09-01 DOI:10.1016/j.angen.2020.200106

Kaouadji Zoubeyda , Meghelli Imane , Cherifi Youcef , Babelhadj Baaissa , Gaouar S.B. Suheil , Conte Michela , Capocefalo Antonio , Agrimi Umberto , Chiappini Barbara , Vaccari Gabriele

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引用次数: 3

Abstract

Prion diseases are a group of animal and human neurodegenerative, albeit transmissible diseases. The lack of effective preventive and therapeutic approaches represents a serious problem in their management. This is especially true for those prion diseases of animals which behave like infectious and contagious diseases. In the case of sheep scrapie, the well- known role of variations in the prion protein gene (PRNP) in conferring resistance/susceptibility represents an opportunity which has been exploited to select populations genetically resistant to the disease.

The recent description of Camel prion disease (CPrD) in Algeria and Tunisia and the suspicion falling into the category of infectious prion diseases, make urgent to investigate the possible existence of genetic determinants useful for its control.

Herein, we investigated PRNP variability in 232 animals from six dromedary populations (Azawad, Hybrid, Naili, Rguibi, Sahraoui, Targui) in Algeria. A Gly69Ser mutation was observed in a single animal of the Targui population and a Gly134Glu polymorphism in the Azawad, Hybrid and Rguibi populations, with a frequency of the 134Glu allele of 2.6%, 7.7% and 7.1%, respectively.

Although our work highlights a low variability of Algerian dromedary PRNP, as a possible indication of a recent evolutionary history of CPrD, it offers also evidence of PRNP variants whose role in prion disease resistance/susceptibility deserve to be deepened.

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阿尔及利亚单峰骆驼种群中朊蛋白基因(PRNP)的变异

朊病毒疾病是一组动物和人类的神经退行性疾病，尽管是传染性疾病。缺乏有效的预防和治疗方法是其管理中的一个严重问题。对于那些表现得像传染病和传染性疾病的动物朊病毒疾病来说尤其如此。在羊痒病的情况下，众所周知，朊蛋白基因(PRNP)变异在赋予抗性/易感性方面的作用代表了一个被利用来选择对该疾病具有遗传抗性的群体的机会。阿尔及利亚和突尼斯最近对骆驼朊病毒病(CPrD)的描述以及怀疑属于传染性朊病毒疾病类别，迫切需要调查是否存在有助于控制该疾病的遗传决定因素。在此，我们调查了来自阿尔及利亚6个单峰种群(Azawad, Hybrid, Naili, Rguibi, Sahraoui, Targui)的232只动物的PRNP变异性。Targui群体中1只动物存在Gly69Ser突变，Azawad、Hybrid和Rguibi群体中存在Gly134Glu多态性，其中Gly134Glu等位基因频率分别为2.6%、7.7%和7.1%。尽管我们的工作强调了阿尔及利亚单峰骆驼PRNP的低变异性，作为CPrD近期进化史的可能指示，它也提供了PRNP变异在朊病毒疾病抗性/易感性中的作用值得深化的证据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Animal Gene Agricultural and Biological Sciences-Insect Science

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期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.