Reactivation of immobilized acetyl cholinesterase in an amperometric biosensor for organophosphorus pesticide

K.C. Gulla, M.D. Gouda, M.S. Thakur, N.G. Karanth
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引用次数: 62

Abstract

Biosensors based on acetyl cholinesterase (AChE) inhibition have been known for monitoring of pesticides in food and water samples. However, strong inhibition of the enzyme is a major drawback in practical application of the biosensor which can be overcome by reactivation of the enzyme for repeated use. In the present study, enzyme reactivation by oximes was explored for this purpose. Two oximes viz., 1,1′-trimethylene bis 4-formylpyridinium bromide dioxime (TMB-4) and pyridine 2-aldoxime methiodide (2-PAM) were compared for the reactivation of the immobilized AChE. TMB-4 was found to be a more efficient reactivator under repeated use, retaining more than 60% of initial activity after 11 reuses, whereas in the case of 2-PAM, the activity retention dropped to less than 50% after only 6 reuses. Investigations also showed that reactivation must be effected within 10 min after each analysis to eliminate the ageing effect, which reduces the efficiency of reactivation.

固定化乙酰胆碱酯酶在有机磷农药安培传感器中的再激活
基于乙酰胆碱酯酶(AChE)抑制的生物传感器已被用于监测食品和水样中的农药。然而,酶的强抑制作用是生物传感器实际应用中的一个主要缺点,这可以通过酶的再激活来克服。在本研究中,酶的再活化由肟探讨了这一目的。比较了1,1′-三亚甲基双4-甲酰基溴化吡啶二肟(TMB-4)和吡啶2-醛肟甲氧基(2-PAM)两种肟类对固定化AChE的再活化作用。在重复使用的情况下,TMB-4被发现是一个更有效的再活化剂,在重复使用11次后保留了60%以上的初始活性,而在2-PAM的情况下,重复使用6次后活性保留率下降到50%以下。调查还表明,每次分析后必须在10分钟内进行再活化,以消除老化效应,这降低了再活化的效率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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