Differenzierungsverhalten und Stoffwechselaktivitäten in Kalluskulturen aus Stolonen und Knollen von Solanum tuberosum

Abstract

Induction of callus formation, growth rate and differentiation pattern of calli derived from potato tuber tissue or from stolons were investigated depending on the hormone content and composition of nutrient media. Media are described giving callus induction, various types of organ differentiations (roots, shoots with or without tubers) and regenerates. Metabolic activity of various tissues was examined by following [¹⁴CO₂]-fixation via the phosphoenol-pyruvate-carboxylase pathway and by studying uptake and metabolisation of U-[¹⁴C]-glucose. On a fresh weight basis, both tissues of intact potato tubers and derived calli showed a rather similar rate and labelling pattern of [¹⁴CO₂]-fixation. Native potato tuber tissue, however, metabolized added U-[¹⁴C]-glucose with a much higher rate than callus tissue. Also, in the former a major part of label from U-[¹⁴C]-glucose was found in sucrose, whereas callus tissue from the same plant material showed little sucrose formation. Starch formation from U-[¹⁴C]-glucose was equally low in both kinds of tissue.