The Cell Wall Glycoprotein of Halobacteria: Structural, Functional and Biosynthetic Aspects

Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie Pub Date : 1982-03-01 DOI:10.1016/S0721-9571(82)80064-1

Felix Wieland , Johann Lechner, Manfred Sumper

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引用次数: 39

Abstract

The cell wall glycoprotein of Halobacteria contains sulphate covalenty bound to different types of saccharides. A high molecular weight saccharide (HMW-saccharide), which is N-glycosidically linked to Asn was found to have the following composition: 1 gal: 1 galN: 1 glcN: 2 galUA: 2 SO₄^2-. This HMW-saccharide is not a heterosaccharide, but exhibits rather a repeating unit structure similar to that of the animal glycosaminoglycans. Incorporation into the cell wall glycoprotein of this HMW-saccharide is specifically inhibited by the antibiotic bacitracin (Wieland et al., 1980). This inhibition results in a remarkable change of the halobacterial shape: the normally rod shaped cells grown in the presence of bacitracin convert to regular spheres (Mescher and Strominger, 1975).

This HMW-saccharide is synthesized bound to a lipid anchor: only after completion the fully sulphated saccharide is transferred to the core protein.

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盐杆菌细胞壁糖蛋白:结构、功能和生物合成方面

盐杆菌的细胞壁糖蛋白含有与不同类型的糖结合的硫酸盐共价。n -糖苷连接Asn的一种高分子量糖(hmw - sugar)，其组成为:1 gal: 1 galN: 1 glcN: 2 galUA: 2 SO42-。这种高分子量糖不是一种杂糖，而是一种类似于动物糖胺聚糖的重复单元结构。这种高分子量糖与细胞壁糖蛋白的结合被抗生素杆菌肽特异性抑制(Wieland et al.， 1980)。这种抑制导致盐杆菌形状的显著变化:在杆菌肽存在下生长的正常棒状细胞转变为规则的球体(Mescher和Strominger, 1975)。这种高分子量糖被合成到一个脂质锚点上:只有在完成后，完全硫酸化的糖才被转移到核心蛋白上。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie

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