Two DFSA cases involving midazolam clarified by the micro-segmental hair analyses.

IF 2.8 4区医学 Q2 TOXICOLOGY

Forensic Toxicology Pub Date : 2022-07-01 DOI:10.1007/s11419-022-00621-1

Duoqi Xu, Jiaojiao Ji, Ping Xiang, Hui Yan, Min Shen

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引用次数: 5

Abstract

Purpose: In this study, an analytical procedure to identify trace amounts of drug in hair based on micro-segmental hair analysis was presented. The method also can be used to estimate the time of drug ingestion at daily precision by cutting a single hair into sub-millimeter segments which correspond to daily hair growth.

Methods: A method was established for efficient extraction of midazolam, one of the most frequently detected compound in drug-facilitated sexual assault (DFSA) cases, from each 0.4-mm hair segment and validated by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Moreover, two DFSA cases were used to compare the micro-segmental hair analysis with the 1- cm segmental analysis method.

Results: The validation showed a lower limit of quantification of 0.5 pg/mm for midazolam, with intraday and interday accuracies (bias) from - 5.2 to 0.9%. The micro-segmental hair analysis method was applied to proximal 1-cm hair segment including hair bulbs in two DFSA cases. The micro-segmental hair analysis results in case 1 showed midazolam in the S15-S17 (5.6-6.8 mm from hair bulb) in a concentration range from 0.5 to 0.9 pg/mm, and the concentrations of midazolam in all hair micro-segments (0-1 cm from the scalp) in case 2 were from 0.5 to 2.0 pg/mm.

Conclusions: Comparison with the conventional method revealed that micro-segmental hair analysis may enhance the utility of hair drug testing and strengthen probative force in DFSA cases.

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两个DFSA病例涉及咪达唑仑澄清微节段头发分析。

目的:建立一种基于毛发微节段分析的毛发中微量药物的分析方法。该方法还可以通过将一根头发切割成亚毫米级的片段来估计药物摄入的时间，以达到每日的精确度。方法:建立了一种从0.4 mm毛段中高效提取药物性侵(DFSA)案件中检出最多的化合物之一咪达唑仑的方法，并采用超高效液相色谱-串联质谱(UPLC-MS/MS)进行验证。并以2例DFSA病例为例，比较微节段毛发分析与1- cm节段毛发分析方法。结果:咪达唑仑的定量下限为0.5 pg/mm，日内和日间准确度(偏差)为- 5.2 ~ 0.9%。应用微节段毛发分析方法对2例DFSA患者近端1 cm的包括毛球的毛发节段进行分析。病例1的微节段头发分析结果显示，S15-S17(距毛球5.6-6.8 mm)的咪达唑仑浓度范围为0.5 - 0.9 pg/mm，病例2的所有头发微节(距头皮0-1 cm)的咪达唑仑浓度范围为0.5 - 2.0 pg/mm。结论:与常规方法相比，微片段毛发分析可提高头发药物检测的实用性，增强DFSA病例的证明力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Forensic Toxicology TOXICOLOGY-

CiteScore

5.80

自引率

9.10%

发文量

审稿时长

3 months

期刊介绍： The journal Forensic Toxicology provides an international forum for publication of studies on toxic substances, drugs of abuse, doping agents, chemical warfare agents, and their metabolisms and analyses, which are related to laws and ethics. It includes original articles, reviews, mini-reviews, short communications, and case reports. Although a major focus of the journal is on the development or improvement of analytical methods for the above-mentioned chemicals in human matrices, appropriate studies with animal experiments are also published. Forensic Toxicology is the official publication of the Japanese Association of Forensic Toxicology (JAFT) and is the continuation of the Japanese Journal of Forensic Toxicology (ISSN 0915-9606).