In vivo anti-plasmodial activity and histopathological analysis of water and ethanol extracts of a polyherbal antimalarial recipe

Journal of Pharmacognosy and Phytotherapy Pub Date : 2017-06-30 DOI:10.5897/JPP2017.0449

M. R. Ibukunoluwa

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引用次数: 9

Abstract

Anthocleista djalonensis A. Chev. (stem bark), Azadirachta indica A. Juss (stem bark and leaf), Cajanus cajan (L.) Huth. (leaf), Crescentia cujete L. (stem bark), Lawsonia inermis L. (leaf), Lophira alata Banks ex C.F. Gaertn. (stem bark), Myrianthus pruessii Engl. (leaf), Nauclea latifolia Sm. (stem bark), Olax subscorpioidea Oliv. (root), and Terminalia glaucescens Planch ex Benth. (stem bark and root) are combined for use in the treatment of malaria in Akure, Southwestern Nigeria. The powdered plant samples were screened for phytochemical constituents, proximate composition and mineral elements according to standard protocols. Plasmodium berghei infected mice were screened for parasitemia and administered with water and ethanol extracts of the combined plant sample. Toxicity and histopathological studies were carried out on the liver and kidney sections of the mice. Data were statistically analyzed. The powdered herbal recipe contained appreciable phytochemicals and important minerals. The concentrations administered for LD50 did not elicit adverse reactions in the experimental animals, and no mortality was recorded. Histological studies revealed some pathology caused by the malaria parasite, as well as side effects of the extracts administered. This is discussed in relation to safety considerations. Key words: Malaria, herbs, phytochemical, histopathology, Nigeria.

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抗疟复方水提取物和乙醇提取物的体内抗疟原虫活性及组织病理学分析

贾龙骨Anthocleista . Chev。(茎皮)，印楝(茎皮和叶)，Cajanus cajan (L.)胡特。(叶)，月牙(茎皮)，月牙(叶)，枇杷(叶)。(茎皮)，Myrianthus pruessii Engl。(叶);核仁;(茎树皮)，橄榄科近蝎总橄榄。(根)，和glaucescens Terminalia Planch前底。在尼日利亚西南部的阿库雷，(茎、树皮和根)被结合起来用于治疗疟疾。根据标准方案对粉末状植物样品进行植物化学成分、近似成分和矿物元素的筛选。筛选伯氏疟原虫感染小鼠的寄生虫血症，并给予联合植物样品的水和乙醇提取物。对小鼠肝、肾切片进行毒性和组织病理学研究。对数据进行统计学分析。粉末草药配方含有可观的植物化学物质和重要的矿物质。给药浓度的LD50在实验动物中没有引起不良反应，也没有死亡记录。组织学研究揭示了疟疾寄生虫引起的一些病理，以及所施用提取物的副作用。这是与安全考虑有关的讨论。关键词:疟疾，草药，植物化学，组织病理学，尼日利亚

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来源期刊

Journal of Pharmacognosy and Phytotherapy

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