Correlation Analysis of Drug Resistance and Integron Gene Types of Food-borne Salmonella from Jilin Province

Abstract

Objective: To detec integron genes and the drug resistance of two foodborne salmonella from different sources in Jilin Province, explore the correlation between drug resistance and integron. Method: The minimum inhibitory concentration (MIC) method was used to detect drug resistance of 278 salmonella strains in Jilin Province. Real-time PCR was used to detect type I, II and III integron genes, and the correlation between drug resistance and integron gene carrier rate was compared. Result: The total drug resistance rate of 278 strains of food-borne Salmonella was 89.57% (249/278). The positive rates of type I integron, type II integron and type III integron were 51.44% (143/278), 4.32% (12/278) and 14.39% (40/278) respectively. The drug resistance rate of foodborne disease samples was 95.8% (207/216), type I integron was 58.8% (127/216), type II integron was 5.56% (12/216), and type III integron gene was 17.59% (38/216). The drug resistance rate of food (meat, egg) samples was 67.7% (42/62), type I integron was 25.81% (16/62), type III integron gene was 3.23% (2/62), and type II integron gene was detected. Conclusion: Through the surveillance of Salmonella drug resistance and Integron gene carrying rate in food safety risk surveillance and food-borne disease surveillance in Jilin Province, The gene carrying rate of type I and III Integron of Salmonella from food-borne diseases was significantly higher than that of food (meat and eggs). Integron gene system plays an important role in drug resistance transmission of foodborne pathogens. It is suggested that attention should be paid to the monitoring of salmonella drug resistance to ensure food safety and human health.