Zinc deficiency exacerbates loss in blood-brain barrier integrity induced by hyperoxia measured by dynamic MRI.

Michael D. Noseworthy, Tammy M. Bray
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引用次数: 32

Abstract

Using dynamic Magnetic Resonance Imaging (dMRI), blood-brain barrier (BBB) permeability (k(PSrho)) and tissue interstitial leakage space (v(e)) were evaluated in zinc-deficient (ZnDF) male weanling Wistar rats following 3 days exposure to hyperoxia (85% O2). Temporal monitoring of T1-weighted MR image changes, following a bolus intravenous injection of gadolinium-DTPA, allowed estimation of BBB integrity. Three-day exposure of hyperoxia caused a marginal loss of BBB integrity, reflected in a slight increase in kPSrho and v(e), observed in both the animals fed adequate zinc (ZnAL) and pair-fed controls (ZnPF). However, zinc deficiency resulted in a significant increase in both kPSrho and v(e), indicating a severely disturbed BBB. In addition MR-visible free water was elevated in ZnDF brains following hyperoxia treatment indicating that a loss of BBB integrity may be associated with neuronal edema. The diminished BBB integrity may be free-radical mediated as the ratio of oxidized to reduced glutathione (GSSG:GSH) was significantly elevated.
锌缺乏加剧了血脑屏障完整性的丧失,由高氧引起的动态MRI测量。
采用动态磁共振成像(dMRI)技术,对锌缺乏(ZnDF)雄性断奶Wistar大鼠暴露于高氧(85% O2) 3天后的血脑屏障(BBB)通透性(k(PSrho))和组织间质渗漏间隙(v(e))进行了评价。在静脉注射钆- dtpa后,对t1加权MR图像变化进行时间监测,可以估计血脑屏障的完整性。三天的高氧暴露导致血脑屏障完整性的轻微损失,反映在kPSrho和v(e)的轻微增加上,在喂食充足锌(ZnAL)和配对喂食对照(ZnPF)的动物中都观察到。然而,锌缺乏导致kPSrho和v(e)显著增加,表明血脑屏障受到严重干扰。此外,高氧治疗后,ZnDF脑中mr可见的游离水升高,表明血脑屏障完整性的丧失可能与神经元水肿有关。血脑屏障完整性的降低可能是自由基介导的,因为氧化谷胱甘肽与还原性谷胱甘肽(GSSG:GSH)的比例显著升高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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