Escherichia coli as Possible Agents of Spread of Multidrug Resistance in Port Harcourt, Rivers State.

K. Otokunefor, Victor Ogechi Osogho, C. Nwankwo
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引用次数: 3

Abstract

Abstract Multidrug resistance (MDR) continues to be a growing global issue. The problem of MDR is fuelled in part by the spread of the genes encoding resistance horizontally which is linked particularly to conjugation involving plasmids. Studies have demonstrated the presence of plasmids in drug resistant isolates, few have shown a link between these plasmids and drug resistance via plasmid curing especially in our locale. This study set out to explore this link in Escherichia coli isolates from Port Harcourt, Nigeria. Plasmid curing was done on a selection of clinical and non-clinical bacteria using acridine orange and antibiotic susceptibility testing carried out on both cured and uncured variants. Data generated was analysed to ascertain the multiple antibiotic resistance (MAR) index and MDR of each isolate. Data was then compared to ascertain effects of plasmid curing on antibiotic resistance of the isolates. Results revealed a decrease in resistance to 7 of 8 antibiotics following plasmid curing. The highest change was noted in ceftazidime (40%), followed by ofloxacin (26.7%). Plasmid curing caused a shift in MAR index values of isolates from higher to lower indices. At MAR index values of ≤0.25 occurrence increased from 5% to 36.7% while at MAR index values ≥0.75, occurrence reduced from 29.9% to 10.0%. A reduction in the degree of MDR was noted (from 55% to 36.7%). Strikingly, the reduction in MDR level of non-clinical isolates was 30% as opposed to 3.4% in the clinical isolates. This study shows a link between plasmids and antibiotic resistance. For the non-clinical isolates, the high-level link between MDR and plasmid carriage could indicate a higher use of antimicrobials in non-clinical rather than clinical settings. Additionally, it could be an indicator for a higher risk of the transfer of MDR determinants from non-clinical sources to human populations in our locale.
大肠杆菌是河流州哈科特港多药耐药传播的可能媒介。
多药耐药(MDR)是一个日益严重的全球性问题。耐多药问题的部分原因是编码耐药性的基因水平传播,特别是与涉及质粒的结合有关。研究表明,在耐药分离株中存在质粒,但很少有研究表明这些质粒与通过质粒固化产生耐药性之间存在联系,特别是在我国。本研究的目的是在尼日利亚哈科特港分离的大肠杆菌中探索这种联系。使用吖啶橙对选择的临床和非临床细菌进行质粒固化,并对固化和未固化的变体进行抗生素敏感性测试。对产生的数据进行分析,确定每个分离株的多重抗生素耐药指数和MDR。然后对数据进行比较,以确定质粒固化对分离株抗生素耐药性的影响。结果显示,质粒固化后,对8种抗生素中7种的耐药性下降。头孢他啶变化最大(40%),其次是氧氟沙星(26.7%)。质粒固化导致分离株的MAR指数由高指数向低指数转变。在MAR指数≤0.25时,发生率从5%上升到36.7%,在MAR指数≥0.75时,发生率从29.9%下降到10.0%。MDR程度有所降低(从55%降至36.7%)。引人注目的是,非临床分离株的MDR水平下降了30%,而临床分离株的MDR水平下降了3.4%。这项研究显示了质粒和抗生素耐药性之间的联系。对于非临床分离株,耐多药和质粒携带之间的高水平联系可能表明,在非临床而非临床环境中使用了更多的抗微生物药物。此外,这可能是耐多药决定因素从非临床来源向我们地区人群转移风险较高的一个指标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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