Genotoxic Effects of Aluminium Acetate by Micronucleus Assay

Abstract

Aluminium, the mostly used element in the world, and its usage is increasing in many applications. Aluminium acetate (AA) has been used in many therapeutical applications, too. Because of widely usage of AA in medicine, its safety must be clearly defined. This research evaluated the genotoxic effects of AA. It has been known that aluminium had very important toxic effect in long time exposures. It was aimed to determine if AA had same genotoxic effects or not. Genotoxic effects of AA were determined with human lymphocytes MN assay. Control, Negative control (1% DMSO), positive control (MMC, 0.20 µg/ml), 1250, 2500, 5000, 10000, and 15000 ppm doses of AA were used in this assay. MNBC % and NDI values of AA were compared with negative control group. 15000 ppm of AA were observed as EC50 concentration that reduced the mitotic index about 50 %. As a result of MNBC %,  there was statistically significant increase in MN frequency at 5000 and 10000 ppm doses.  Dose-dependent cytotoxicity were obtained from NDI study. The highest MN frequency and the lowest NDI frequency were obtained from 10000 ppm at 48 h treatment while the lowest MN and the highest NDI frequencies were determined in 1250 ppm at 24 h treatment. This research showed that used concentrations did not produce significant MN frequency but significantly reduced the NDI value when compared to MMC.