PALYNOMORPHOLOGICAL STUDY OF WEEDY MELLIFEROUS (BEE VISITED) PLANTS USING LIGHT MICROSCOPIC TECHNIQUES FROM SOUTHERN KHYBER PAKHTUNKHWA, PAKISTAN

F. Khan, K. Khan, Shabir Ahmad
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Abstract

Pollen morphology of 10 different weedy bee foraged plants belong to 10 various families from Southern Khyber Pakhtunkhwa were collected, identified and studied using light microscopy (LM). The plants were Asphodelus tenuifolius, Euphorbia helioscopia, Parthenium hysterophorus, Rhazya stricta, Datura innoxia, Eruca sativa, Convolvulus arvensis, Anagallis arvensis, Galium aparine, and Anethum graveolens. Slides for Light microscopic studies were prepared with the help of acetic acid, glycerin jelly and anthers of flowers. Pollen grain recorded ranged from monocolpate to hexacolporate and from psilate to echinate which were important systematic significance. Pollen size, shape, P/E ratio, exine thickness, number of colpi, number of pores, equatorial diameter, polar diameter, colpus width, colpus length, spines number, length and width of spines were examined with the help of light microscopy and all these values were analyzed statistically using software SPSS. This research provide a data to the optimal utilization of bee foraged weed plants by honeybees and identification of bee flora for the beekeeping business and honey production. Results revealed that weedy melliferous flora of study area is very helpful for botanical origin, geographical origin of bee species and adulterations found in honey.
巴基斯坦开伯尔-普赫图赫瓦南部禾本科植物的光显微形态学研究
对开伯尔-普赫图赫瓦省南部10科10种不同的杂草蜂采食植物的花粉形态进行了光镜鉴定和研究。这些植物分别是:细叶马尾松、大戟、蛇耳草、拉子草、曼陀罗、紫花苜蓿、旋花草、蛇耳草、猪耳草、茴香草。用乙酸、甘油果冻和花的花药制备了光镜研究用的载玻片。记录的花粉粒从单胚轴到六胚轴,从棘胚轴到棘胚轴,具有重要的系统意义。光镜下检测花粉大小、形状、P/E比、外壁厚度、柱头数、气孔数、赤道径、极径、柱头宽、柱头长、棘数、棘长、棘宽,并用SPSS软件进行统计分析。本研究为蜜蜂对蜂采杂草植物的优化利用和蜂区系的鉴定提供了依据,为养蜂业和蜂蜜生产提供了依据。结果表明,研究区杂草类植物区系对植物来源、蜜蜂种类地理来源和蜂蜜掺假有重要的参考价值。
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