Phytochemical and in vitro thrombolytic activity evaluation of Cassia siamea L., Leguminosae leaf extracts, and pyrogallol

M. Vani
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引用次数: 1

Abstract

Aim: The aim of the study was to carry out total phenolic content determination followed by in vitro thrombolytic studies of aqueous and alcoholic extracts of Cassia siamea L., extracts and pyrogallol. Materials and Methods: The plant leaf aqueous and alcoholic extractive values, preliminary phytochemical screening followed by total phenolic content was determined. Further, the plant extracts and one of the plant active constituents’ pyrogallol were studied for in vitro thrombolytic activity using streptokinase (SK) as standard in human venous blood. Results and Discussion: The extractive values were found to be 15.0 and 13.2 w/w for aqueous and alcoholic extracts. The plant extracts showed the presence of phenolics and flavonoids. The total phenolic components present in aqueous and alcoholic extracts were found to be 3 mg/g and 0.3 mg/g equivalent of pyrogallol. The aqueous extract contains more amount of phenolic substances than alcoholic extract. The percentage clot lysis was found to be 2.25% in standard SK, 14.50% in aqueous extract, 18% in the ethanolic extract, and 17.75% in pyrogallol, respectively. The ethanolic extract exhibited better thrombolytic activity than aqueous extract. Pyrogallol also exhibited equivalent thrombolytic activity compared to standard. Conclusion: The results indicate that ethanolic extract exhibited thrombolytic activity which may be due to hydrolysable tannin pyrogallol. Further studies are required to isolate the responsible component from the ethanolic extract and pyrogallol can be studied for mechanism of action studies
决明子、豆科植物叶提取物和邻苯三酚的植物化学和体外溶栓活性评价
目的:测定决明子水提液、醇提液、邻苯三酚的总酚含量,并进行体外溶栓研究。材料与方法:对植物叶片进行水提液和醇提液的提取,初步筛选后测定总酚含量。此外,以链激酶(SK)为标准,研究了植物提取物和其中一种活性成分邻苯三酚在人静脉血中的体外溶栓活性。结果与讨论:水提液和醇提液的提取值分别为15.0和13.2 w/w。植物提取物中含有酚类物质和类黄酮。发现在水和醇提取物中存在的总酚成分分别相当于邻苯三酚3 mg/g和0.3 mg/g。水提物比醇提物含有更多的酚类物质。血块溶解率在标准SK中为2.25%,在水提物中为14.50%,在乙醇提物中为18%,在邻苯三酚中为17.75%。乙醇提取物的溶栓活性优于水提取物。与标准相比,邻苯三酚也表现出相同的溶栓活性。结论:乙醇提取物具有溶栓活性,其作用可能与邻苯三酚的单宁水解有关。需要进一步研究从乙醇提取物中分离出有效成分,并对邻苯三酚进行作用机理研究
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