Effect of Therapy on Mangosteen (Garcinia mangostana L.) Bark Extract on Serum Blood Blood Protease Activity and Expression of Malondialdehyde (MDA) on Rattus norvegicus Traumatic Brain Injury Model

Abstract

Traumatic brain injury is a cause of impaired cognitive and physical function that is permanent or temporary and is accompanied by loss or change of level of consciousness and cause damage or death of cells in the brain. Cell death or apoptosis can cause inflammation and oxidative stress andproduce reactive oxygen species (ROS). The use of mangosteen peel extract serves to prevent inflammation and inhibit the production of oxidative stress. This study aims to determine the role of mangosteen peel extract therapy in brain organ expression and protease activity in rat blood serum. Besides that, minocycline is used as a test standard and comparison of mangosteen peel extract. The results of this study formed 4 treatment groups namely the negative group, the positivegroup TBI model, the TBI group with mangosteen skin extract therapy dose 0.5 mL/day for 5 daysand the TBI group with minocycline therapy 0.5 mL/day dose for 5 days. The results showed that the treatment of mangosteen peel extract after brain injury could reduce the expression ofmalondialdehyde (MDA) by 32.29% and protease activity in rat blood serum by 47.62%. Theresults of statistical analysis of MDA exposure and protease activity showed that there were verysignificant differences between treatment groups (p <0.01). This study can be concluded thatmangosteen peel extract therapy can be used when a traumatic brain injury occurs.