The antimicrobial effect of doxycycline and doped ZnO in TiO2 nanotubes synthesized on the surface of orthodontic mini-implants

Abstract

Anchorage preservation is crucial in orthodontic treatment success. Mini-implants make a revolution in this domain. The failure of orthodontic mini-implants due to inflammation and infection is one of the reasons for anchorage loss. The purpose of this study was to evaluate the effect of a novel mini-implant surface modification to improve resistance against microbial contamination and surrounding tissue inflammation. Twenty-four orthodontic mini-implants (Jeil Medical Corporation, Korea) with 1.6 mm diameter and 8 mm length were randomly divided into three groups: Group 1: Control group, Group 2: Nanotubes were made on the surface with anodisation, and Group 3: Zinc Oxide (ZnO) doped into nanotubes, and then doxycycline is added to them. The anti-bacterial efficacy against Porphyromonas gingivalis was evaluated using the disk diffusion method. To analyze data, Kruskal–Wallis, Friedman, and Wilcoxon tests were done. The significance level was set at 0.05. No zone of the inhibition was formed in Groups 1 and 2. In Group 3, the mean (SD) diameter of the inhibition zone in the first 5-day to sixth 5-day were 38.7(8.2), 25(4.8), 17.8(5.6), 7.63(5.37), 1.5(2.83), and 0 millimeters, respectively. Nanotubes containing doped ZnO and Doxycycline are capable of preventing bacterial growth around the mini implant surfaces for at least up to 30 days. To manage inflammation of surrounding tissues of mini-implants, nanotubes are not effective alone. Therefore, the presence of diffusible materials in addition to nanotubes on the surface of mini-implants is necessary.