Application of Allele-Specific (AS-PCR) Marker for Identification of High-Molecular-Weight Glutenin Subunits (HMW-GS) at the GluB-1 Locus in Bread Wheat (Triticum aestivum L.)

Abstract

Improved processing quality is an important objective of wheat breeding which is largely associated with the amount and composition of endosperm seed proteins in the wheat kernel. Especially, HMW-GS determines dough strength and visco-elasticity contributing towards end-use product. Glu-B1 is the candidate structural gene, coding for Glu1Bx and associated with the quantity of high-molecular-weight (HMW) glutenin in bread wheat. This study provides detection of GluB-1 alleles on 19 different wheat cultivars by precise AS-PCR marker with the aim to develop marker for HMW-GS 1Bx20 which is assumed to be associated with chapatti making quality (CMQ) in bread wheat. The primer pair gave diagnostic banding pattern in different varieties with 1Bx7, 1Bx20 and 1Bx17+18 GluB-1 alleles. The result of this study will help in detection of varieties with different allelic combinations at their GluB-1 loci in early developmental stage using single marker which reduces the time for varietal selection. It will be also useful in screening of wheat cultivars with good chapatti making quality and introgression of subunit 1Bx20 in high yielding varieties using marker-assisted selection (MAS).