Antioxidant Potential of Cell Wall Polysaccharides Extracted from Various Parts of Aerva javanica

Free Radicals and Antioxidants Pub Date : 2019-04-01 DOI:10.5530/FRA.2019.1.7

Hina Saeed, H. Nawaz, M. Shad, D. Shahwar, H. Andaleeb, S. Muzaffar, R. Jabeen, T. Rehman, A. Waheed

{"title":"Antioxidant Potential of Cell Wall Polysaccharides Extracted from Various Parts of Aerva javanica","authors":"Hina Saeed, H. Nawaz, M. Shad, D. Shahwar, H. Andaleeb, S. Muzaffar, R. Jabeen, T. Rehman, A. Waheed","doi":"10.5530/FRA.2019.1.7","DOIUrl":null,"url":null,"abstract":"Cite this article: Saeed H, Nawaz H, Shad MA, Shahwar DE, Andaleeb H, Muzaffar S, et al. Antioxidant Potential of Cell Wall Polysaccharides Extracted from Various Parts of Aerva javanica. Free Radicals and Antioxidants. 2019;9(1):35-42. ABSTRACT Objectives: Aerva javanica, an underutilized desert plant, is a rich source of polysaccharide but not investigated properly for the antioxidant potential of its polysaccharides. The antioxidant potential of cell wall polysaccharides in leaves, branches, stem and root of A. javanica was determined. Methods: In a sequential extraction procedure, the water-soluble fraction (WSF), sodium acetate-EDTA soluble fraction (SASF), sodium carbonate soluble fraction (SCSF), 4% potassium hydroxide soluble fraction (4%KOHSF) and 14% potassium hydroxide soluble fraction (14%KOHSF) were obtained and subjected to antioxidant analysis. Results: The addition of salt or alkali in the extraction media resulted in a significant increase in total extractable polysaccharide content. The 14%KOHSF showed higher extract yield, total antioxidant activity, ferrous ion chelating activity, ferric reducing antioxidant power and linoleic acid reduction capacity. The WSF showed higher scavenging capacity against DPPH radical. WSF of branches and root and SCSF of leaf and stem showed highest scavenging capacity against hydroxyl radical. Conclusion: The lower extraction yield but high radical scavenging capacity of WSF may be attributed to the presence of some protein linked hydrophilic polysaccharides of pectin nature. The high extractable polysaccharide content with strong antioxidant potential makes A. javanica a suitable candidate for the antioxidant polysaccharide based pharmaceutical and industrial applications.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"32 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Free Radicals and Antioxidants","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5530/FRA.2019.1.7","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 2

Abstract

Cite this article: Saeed H, Nawaz H, Shad MA, Shahwar DE, Andaleeb H, Muzaffar S, et al. Antioxidant Potential of Cell Wall Polysaccharides Extracted from Various Parts of Aerva javanica. Free Radicals and Antioxidants. 2019;9(1):35-42. ABSTRACT Objectives: Aerva javanica, an underutilized desert plant, is a rich source of polysaccharide but not investigated properly for the antioxidant potential of its polysaccharides. The antioxidant potential of cell wall polysaccharides in leaves, branches, stem and root of A. javanica was determined. Methods: In a sequential extraction procedure, the water-soluble fraction (WSF), sodium acetate-EDTA soluble fraction (SASF), sodium carbonate soluble fraction (SCSF), 4% potassium hydroxide soluble fraction (4%KOHSF) and 14% potassium hydroxide soluble fraction (14%KOHSF) were obtained and subjected to antioxidant analysis. Results: The addition of salt or alkali in the extraction media resulted in a significant increase in total extractable polysaccharide content. The 14%KOHSF showed higher extract yield, total antioxidant activity, ferrous ion chelating activity, ferric reducing antioxidant power and linoleic acid reduction capacity. The WSF showed higher scavenging capacity against DPPH radical. WSF of branches and root and SCSF of leaf and stem showed highest scavenging capacity against hydroxyl radical. Conclusion: The lower extraction yield but high radical scavenging capacity of WSF may be attributed to the presence of some protein linked hydrophilic polysaccharides of pectin nature. The high extractable polysaccharide content with strong antioxidant potential makes A. javanica a suitable candidate for the antioxidant polysaccharide based pharmaceutical and industrial applications.

查看原文本刊更多论文

水芹不同部位细胞壁多糖的抗氧化活性研究

本文来源:Saeed H, Nawaz H, Shad MA, Shahwar DE, Andaleeb H, Muzaffar S，等。水芹不同部位细胞壁多糖的抗氧化活性研究。自由基与抗氧化剂[j] . 2019;9(1):35-42。摘要目的:javanica Aerva是一种未被充分利用的沙漠植物，是多糖的丰富来源，但其多糖的抗氧化潜力尚未得到适当的研究。对爪哇叶、枝、茎、根细胞壁多糖的抗氧化能力进行了测定。方法:采用顺序提取法，提取水溶部分(WSF)、乙酸钠- edta溶部分(SASF)、碳酸钠溶部分(SCSF)、4%氢氧化钾溶部分(4% kohsf)和14%氢氧化钾溶部分(14% kohsf)，并进行抗氧化分析。结果:在提取液中加入盐或碱均可显著提高总可提取多糖含量。14%KOHSF具有较高的提取率、总抗氧化活性、铁离子螯合活性、铁还原抗氧化能力和亚油酸还原能力。WSF对DPPH自由基具有较强的清除能力。对羟基自由基清除能力最强的是枝、根的WSF和叶、茎的SCSF。结论:枸杞多糖提取率低，清除自由基能力强，可能与其含有果胶性质的亲水蛋白多糖有关。爪哇多糖提取率高，具有较强的抗氧化潜力，是抗氧化多糖在制药和工业上的理想选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Free Radicals and Antioxidants

自引率

0.00%

发文量