Host-Range Testing of a Mixture of Two Nucleopolyhedroviruses of Choristoneura fumiferana (Lepidoptera: Tortricidae)

IF 1.1 4区农林科学 Q3 ENTOMOLOGY

Canadian Entomologist Pub Date : 2011-04-01 DOI:10.4039/n10-052

W. Kaupp, Kevin N. Barber, W. Fick, P. Ebling, T. Ladd, S. Holmes

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引用次数: 3

Abstract

Abstract The host range of a mixture of Choristoneura fumiferana (Clemens) nucleopolyhedroviruses (CfMNPV and CfDefNPV) was investigated using a per os bioassay of larvae of 29 species of Lepidoptera and adult males of Megachile rotundata (F.) (Hymenoptera: Megachilidae). Using a whole-genomic DNA probe, positive results were obtained in 8 of 10 Tortricidae: Archips cerasivorana (Fitch), Choristoneura fractivittana (Clemens), C. fumiferana, Choristoneura occidentalis Freeman, Choristoneura pinus pinus Freeman, Choristoneura rosaceana (Harris), Clepsispersicana (Fitch), and Cydia pomonella (L.); one Crambidae: Ostrinia nubilalis (Hübner); one arctiine Erebidae: Estigmene acrea (Drury); and two Noctuidae: Oligia illocata (Walker) and Pyrrhia exprimens (Walker). Mortality rates were highest among C. fumiferana, C occidentalis, C. pinus pinus, A. cerasivorana, and C. pomonella. Sequenced polymerase chain reaction (PCR) amplicons from infected individuals from several species confirmed that the primer sets amplified the target viruses. CfMNPV was consistently found in virus-fed C. fumiferana; whereas, CfDefNPV was present only occasionally. The presence of CfMNPV and CfDefNPV in A. cerasivorana was confirmed by PCR and DNA sequencing. Significant treatment-mortality rates were induced in the noctuids P. exprimens and Acronicta impleta Walker; PCR determined that both viruses were present in treated P. exprimens but only CfMNPV was present in A. impleta. No virus was detected in M. rotundata.

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富米蛾两种核多角体病毒混合物的宿主范围检测(鳞翅目:蛾科)

摘要采用鳞翅目29种幼虫和圆腹大蠊(Megachile rotundata, F.)成年雄虫(膜翅目:大蠊科)的生物测定方法，研究了fumiferana (Clemens)核多角体病毒(CfMNPV和CfDefNPV)的混合寄主范围。利用全基因组DNA探针，在10种蝽科中有8种获得阳性结果，分别是:紫斑蝽(Archips cerasivorana, Fitch)、碎裂绒螯虾(chistoneura fractivitana, Clemens)、fumiferana、西纹绒螯虾(chistoneura occidentalis Freeman)、松纹绒螯虾(chistoneura pinus Freeman)、玫瑰绒螯虾(chistoneura Harris)、紫纹绒螯虾(Clepsispersicana, Fitch)和pomonella Cydia;一蟹科:鸵鸟(Ostrinia nubilalis);一种菊科:残余面积;和两个夜蛾科:夜蛾(Walker)和夜蛾(Pyrrhia)。死亡率最高的是烟熏木蠹、西方木蠹、松木蠹、中国木蠹和pomonella木蠹。来自多个物种的感染个体的测序聚合酶链反应(PCR)扩增结果证实，引物组扩增了目标病毒。CfMNPV在富米氏梭菌中一致存在;而CfDefNPV只是偶尔出现。CfMNPV和CfDefNPV通过PCR和DNA测序证实了其存在。夜蛾(noctuids P.实验)和水蛭(Acronicta impleta Walker)的处理死亡率显著;聚合酶链反应测定这两种病毒均存在于处理过的假单胞虫实验中，但在假单胞虫中只存在CfMNPV。未在圆形圆孢杆菌中检测到病毒。

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来源期刊

Canadian Entomologist 生物-昆虫学

CiteScore

2.20

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： French translation follows/le français suit Published since 1868, this peer-reviewed bimonthly publication is the official journal of the Entomological Society of Canada. Available via the internet and with hardcopy distribution to 55 countries, its research papers and notes are relevant to entomologists and other biologists around the world. In addition to being a venue for topical reviews and forum discussion, The Canadian Entomologist publishes current research in all facets of entomology, including systematics and morphology, molecular and developmental biology, ecology and behaviour, biodiversity and evolution, insect management, entomological techniques, and other relevant subject areas. Contributions are published in English or French. Authors will not pay page charges, and will experience fast, high quality reviews of their papers.Publiée depuis 1868, cette publication bimestrielle avec comité de lecture est la revue officielle de la Société d’entomologie du Canada. Disponible sur Internet et distribuée en format papier dans 55 pays, ses articles de recherche et ses notes sont pertinents pour les entomologistes et autres biologistes de par le monde. En plus d’être une plate-forme pour des revues thématiques et un forum de discussion, The Canadian Entomologist publie la recherche actuelle sur toutes les facettes de l’entomologie, incluant la systématique et la morphologie, la biologie moléculaire et développementale, l’écologie et le comportement, la biodiversité et l’évolution, la gestion des insectes, les techniques entomologiques et d’autres domaines pertinents. Les contributions sont publiées en français ou en anglais. Les auteurs ne paient aucun frais de publication, et recevront une révision rapide et de grande qualité de leurs articles.