Evaluation the Ability to Isolate and Culture Stem Cells from Frozen Human Umbilical Cord Tissue

VNU Journal of Science: Natural Sciences and Technology Pub Date : 2022-06-10 DOI:10.25073/2588-1140/vnunst.5334

N. Nhung, D. T. Kien, N. Hiep, N. Hanh

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Abstract

Umbilical cord-derived mesenchymal stem cells have been determined to be effective in cell therapy and regenerative medicine. Cell cryopreservation is widely used for long term preservation. However, cell cryopreservation is costly and labor consume. Therefore, cryopreservation of human umbilical cord (HUC) tissue has become an alternative solution to reduce the risks of disease and open up opportunities for later treatment for families in recent years. In this study, we evaluated the ability to isolate and the characteristic of mesenchymal stem cells (MSCs) derived from fresh and frozen human umbilical cord tissue. The results showed that the HUCMSCs were isolated successfully from both fresh and frozen HUC tissues. The expression of MSCs cell surface marker CD73, CD90, CD105 in group 1 were higher than those in group 2, CD73 (99,42 vs 95.2%); CD90 (99.74 vs 96.6%); CD105 (99.64 vs 96.15%), respectively. However, they are similar in morphology and osteogenic and adipose differentiated ability. The results of the study would be useful for stem cell research, cell therapy and regenerative medicine.

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评价从冷冻人脐带组织中分离和培养干细胞的能力

脐带间充质干细胞已被证实在细胞治疗和再生医学中是有效的。细胞低温保存被广泛用于长期保存。然而，细胞冷冻保存成本高且耗费人力。因此，近年来，人类脐带(HUC)组织的冷冻保存已成为降低疾病风险的替代解决方案，并为家庭提供了后期治疗的机会。在这项研究中，我们评估了从新鲜和冷冻的人脐带组织中分离间充质干细胞(MSCs)的能力和特性。结果表明，从新鲜和冷冻的HUC组织中均可成功分离到HUC间充质干细胞。MSCs表面标志物CD73、CD90、CD105在1组的表达高于2组(99.2%、42% vs 95.2%);CD90 (99.74 vs 96.6%);CD105 (99.64 vs 96.15%)。然而，它们在形态和成骨和脂肪分化能力上是相似的。该研究结果对干细胞研究、细胞治疗和再生医学具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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VNU Journal of Science: Natural Sciences and Technology

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