Effect of varying the concentration of sulphuric acid and ammonium hydroxide on the release of cellulose from isolated cell wall component of corn cob

Cameroon Journal of Experimental Biology Pub Date : 2021-10-04 DOI:10.4314/cajeb.v14i2.1

S. Yakubu, Ya’u Anas, Halima Ibrahim, Fati Ahmed Abdullahi, A. S. Dalhatu

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Abstract

The scarcity and high price associated with fossil fuel has urged countries to research resources for alternative energy sources. Biofuels like bioethanol produced from lignocellulosic biomass (corn cob) were considered potential alternative. Cellulose composition from isolated cell wall material of corn cobs was investigated under two different pre-treatments using H2SO4 and NH4OH at varying concentrations of 5%, 10%, 20%, 30% and 40%. Cell wall not treated acted as control. Colorimetric anthrone-assay followed by absorbance reading at 625nm revealed that glucose is present in reasonable amount in corn cob. The analysis of variance (ANOVA) indicated significant differences among pre-treated compared to untreated (Control) corn cob samples at p≤0.05. Acid pre-treatment showed better glucose yield compared to alkali pre-treatment with results revealing 20% (19.37µg/ml) H2SO4 to be the optimal concentration producing highest glucose yield. The study reveals the potential of corn cob as a lignocellulosic feed stock for biofuel production.

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不同浓度的硫酸和氢氧化铵对玉米芯分离细胞壁组分纤维素释放的影响

化石燃料的稀缺性和高价格促使各国研究替代能源资源。从木质纤维素生物质(玉米芯)中生产的生物乙醇等生物燃料被认为是潜在的替代品。采用浓度分别为5%、10%、20%、30%和40%的H2SO4和NH4OH进行预处理，研究了玉米芯细胞壁材料中纤维素的组成。未处理的细胞壁作为对照。比色蒽酮测定和625nm吸光度读数显示，玉米芯中葡萄糖含量合理。方差分析(ANOVA)显示，预处理玉米芯样品与未处理玉米芯样品(对照)的差异显著(p≤0.05)。与碱预处理相比，酸预处理的葡萄糖产率更高，结果表明20%(19.37µg/ml) H2SO4的最佳浓度可产生最高的葡萄糖产率。这项研究揭示了玉米芯作为生物燃料生产的木质纤维素原料的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Cameroon Journal of Experimental Biology

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