miR-19-3p Targets PTEN to Regulate Cervical Cancer Cell Proliferation, Invasion, and Autophagy.

IF 1.4 4区生物学 Q4 GENETICS & HEREDITY

Genetics research Pub Date : 2023-01-01 DOI:10.1155/2023/4784500

Wei Wang, Lu Liu, Yongjian Tian

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引用次数: 2

Abstract

Background: Cervical cancer is the second most common cancer among women worldwide. Extensive studies have shown that microRNAs (miRNA/miR) can regulate the formation, progression, and metastasis of cancer. The purpose of this study was to investigate the effect of miR-19-3p on the proliferation, invasion, and autophagy of cervical cancer cells and to explore the underlying mechanism.

Methods: SiHa and HeLa cells were transfected with miR-19-3p mimic and inhibitor. miR-19-3p and PTEN expression were detected using real-time quantitative PCR and western blot, respectively. The binding between miR-19-3p and PTEN was predicted using Targetscan7.2 and verified by a dual-luciferase reporter gene assay. The effects of miR-19-3p on cell invasion and proliferation were evaluated by Transwell assays and MTT, respectively. The effect of miR-19-3p on autophagy was observed using fluorescence microscopy.

Results: The expression of miR-19-3p in cervical cancer tissues and SiHa and HeLa cells was significantly upregulated, whereas the expression of PTEN was significantly downregulated. PTEN was one of the direct targets of miR-19-3p. The miR-19-3p mimic significantly reduced the apoptosis rate and autophagy and promoted cell proliferation and invasion of the SiHa and HeLa cells.

Conclusion: In summary, miR-19b-3p can target PTEN to regulate the proliferation, invasion, and autophagy of cervical cancer cells. Our findings indicate the potential of miR-19-3p as a target for cervical cancer treatment in the future.

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miR-19-3p靶向PTEN调控宫颈癌细胞增殖、侵袭和自噬

背景:宫颈癌是世界范围内女性中第二常见的癌症。大量研究表明，microRNAs (miRNA/miR)可以调控癌症的形成、进展和转移。本研究旨在探讨miR-19-3p对宫颈癌细胞增殖、侵袭和自噬的影响，并探讨其可能的机制。方法:用miR-19-3p模拟物和抑制剂转染SiHa和HeLa细胞。采用实时定量PCR和western blot分别检测miR-19-3p和PTEN的表达。使用Targetscan7.2预测miR-19-3p与PTEN之间的结合，并通过双荧光素酶报告基因试验进行验证。采用Transwell法和MTT法分别评价miR-19-3p对细胞侵袭和增殖的影响。荧光显微镜观察miR-19-3p对细胞自噬的影响。结果:miR-19-3p在宫颈癌组织及SiHa、HeLa细胞中表达显著上调，PTEN表达显著下调。PTEN是miR-19-3p的直接靶点之一。miR-19-3p mimic显著降低SiHa和HeLa细胞的凋亡率和自噬，促进细胞增殖和侵袭。结论:综上所述，miR-19b-3p可靶向PTEN调控宫颈癌细胞的增殖、侵袭和自噬。我们的研究结果表明miR-19-3p在未来有可能成为宫颈癌治疗的靶点。

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来源期刊

Genetics research 生物-遗传学

自引率

6.70%

发文量

审稿时长

>12 weeks

期刊介绍： Genetics Research is a key forum for original research on all aspects of human and animal genetics, reporting key findings on genomes, genes, mutations and molecular interactions, extending out to developmental, evolutionary, and population genetics as well as ethical, legal and social aspects. Our aim is to lead to a better understanding of genetic processes in health and disease. The journal focuses on the use of new technologies, such as next generation sequencing together with bioinformatics analysis, to produce increasingly detailed views of how genes function in tissues and how these genes perform, individually or collectively, in normal development and disease aetiology. The journal publishes original work, review articles, short papers, computational studies, and novel methods and techniques in research covering humans and well-established genetic organisms. Key subject areas include medical genetics, genomics, human evolutionary and population genetics, bioinformatics, genetics of complex traits, molecular and developmental genetics, Evo-Devo, quantitative and statistical genetics, behavioural genetics and environmental genetics. The breadth and quality of research make the journal an invaluable resource for medical geneticists, molecular biologists, bioinformaticians and researchers involved in genetic basis of diseases, evolutionary and developmental studies.