Comparative Analysis of Central Corneal Thickness in Four Fish Models.

IF 1.4 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Zebrafish Pub Date : 2022-12-01 DOI:10.1089/zeb.2022.0016

Jinpeng Li, Juan Du, Aiping Deng, Ziyang Chen, Yusong Guo, Zhongduo Wang

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引用次数: 0

Abstract

To understand the left-right asymmetry of vertebrate eyes, this study measured the central corneal thickness (CCT) of Oryzias curvinotus, Oryzias melastigma, Oryzias latipes, and zebrafish with optical coherence tomography. The results showed that the CCTs were significant differences among different species and groups, even between the right and left eyes of each fish. The values of the CCTs (mean ± SD, μm) for the four species were 104.71 ± 14.49, 61.88 ± 8.63, 64.76 ± 10.36 and 56.96 ± 10.48, respectively. Moreover, comparing the two wild groups of O. curvinotus from Sanya on N18° and Gaoqiao on N21°, the CCT value for the low-latitude group was 104.71 ± 14.49 μm, greater than the high latitude group 76.13 ± 5.70 μm significantly (t-test, p = 0.0001). Lastly, the paired Student's t-test revealed that significant CCT differences existed between the left and right eye for all four species and groups, of which zebrafish and O. melastigma were belonging to the left thicker type in contrast to the others. This study laid a foundation for understanding the causes of the difference in CCT, and also provided possible fish models for human researches on keratomileusis, glaucoma, and other corneal diseases.

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四种鱼类模型中央角膜厚度的比较分析。

为了了解脊椎动物眼睛的左右不对称性，本研究利用光学相干断层成像技术测量了弯曲米米(Oryzias curvinotus)、黑米米(Oryzias melastigma)、阔米米(Oryzias latipes)和斑马鱼的角膜中央厚度(CCT)。结果表明，不同种类和群体之间的cct存在显著差异，甚至在每条鱼的左右眼之间也是如此。4个物种的cct (mean±SD， μm)分别为104.71±14.49、61.88±8.63、64.76±10.36和56.96±10.48。此外，在N18°的三亚野生组和N21°的高桥野生组比较，低纬度组的CCT值为104.71±14.49 μm，显著大于高纬度组的76.13±5.70 μm (t检验，p = 0.0001)。最后，配对学生t检验显示，所有四个物种和群体的左眼和右眼之间存在显著的CCT差异，其中斑马鱼和O. melastigma属于左厚型，而其他物种属于左厚型。本研究为了解CCT差异的原因奠定了基础，也为人类角膜移植、青光眼等角膜疾病的研究提供了可能的鱼类模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Zebrafish DEVELOPMENTAL BIOLOGY-ZOOLOGY

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

3 months

期刊介绍： Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage. Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community. TechnoFish features two types of articles: TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community Zebrafish coverage includes: Comparative genomics and evolution Molecular/cellular mechanisms of cell growth Genetic analysis of embryogenesis and disease Toxicological and infectious disease models Models for neurological disorders and aging New methods, tools, and experimental approaches Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.