Optimization of Nitrogen Sources and Carbon Source for Recombinant Omega-3 Fatty Acid-Containing Biomass Production

Abstract

The compositions of the medium for recombinant Pichia pastoris producing omega-3 fatty acid were determined for maximum biomass production using crude glycerol as a carbon source and ammonium salts as nitrogen sources. The optimization of the medium was done using Box-Behnken a design software with 3 factors, 3 levels, 1 response for an optimal level of crude glycerol, (NH4)2SO4, (NH4)2HPO4. The optimum medium condition was 40 g/L crude glycerol, 3 g/L (NH4)2SO4, and 7 g/L (NH4)2HPO4. The highest biomass in flasks cultivation after 7 days production was 6.064 g/L DCW and 0.1516 g biomass/ g crude glycerol.