Detection of Acetaminophen and Its Glucuronide in Fingerprint by SALDI Mass Spectrometry Using Zeolite and Study of Time-Dependent Changes in Detected Ion Amount

Analytica Pub Date : 2021-08-02 DOI:10.3390/analytica2030008

Toshiki Horikoshi, C. Kitaoka, Yosuke Fujii, T. Asano, Jiawei Xu, T. Fujino

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Abstract

The ingredients of an antipyretic (acetaminophen, AAP) and their metabolites excreted into fingerprint were detected by surface-assisted laser desorption ionization (SALDI) mass spectrometry using zeolite. In the fingerprint taken 4 h after AAP ingestion, not only AAP but also the glucuronic acid conjugate of AAP (GAAP), caffeine (Caf), ethenzamide (Eth), salicylamide (Sala; a metabolite of Eth), and urea were detected. Fingerprints were collected over time to determine how the amounts of AAP and its metabolite changed with time, and the time dependence of the peak intensities of protonated AAP and GAAP was measured. It was found that the increase of [GAAP+H]+ peak started later than that of [AAP+H]+ peak, reflecting the metabolism of AAP. Both AAP and GAAP reached maximum concentrations approximately 3 h after ingestion, and were excreted from the body with a half-life of approximately 3.3 h. In addition, fingerprint preservation was confirmed by optical microscopy, and fingerprint shape was retained even after laser irradiation of the fingerprint. Our method may be used in fingerprint analysis.

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沸石SALDI质谱法检测指纹图谱中对乙酰氨基酚及其葡糖苷类成分及其离子量随时间变化的研究

采用沸石表面辅助激光解吸电离(SALDI)质谱法测定了一种解热药(对乙酰氨基酚，AAP)及其代谢产物的成分。在AAP摄食4 h后的指纹图谱中，不仅有AAP，还有AAP (GAAP)、咖啡因(Caf)、乙酰胺(Eth)、水杨酸酰胺(Sala)的葡萄糖醛酸偶联物;一种Eth的代谢物)和尿素的检测。随着时间的推移采集指纹图谱，确定AAP及其代谢物的量随时间的变化情况，并测量质子化AAP和GAAP峰强度的时间依赖性。我们发现[GAAP+H]+峰值的增加开始晚于[AAP+H]+峰值，反映了AAP的代谢。AAP和GAAP均在摄入后约3 h达到最大浓度，半衰期约为3.3 h排出体外。此外，光学显微镜证实指纹保存，即使激光照射指纹，指纹形状仍保持不变。该方法可用于指纹分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytica

CiteScore

1.80

自引率

0.00%

发文量