Comparative in vivo and in vitro postmortem ultrasound assessment of intima-media thickness with additional histological analysis in human carotid arteries

Perspectives in Medicine Pub Date : 2012-09-01 DOI:10.1016/j.permed.2012.02.050

Szabolcs Farkas , Sándor Molnár , Katalin Nagy , Tibor Hortobágyi , László Csiba

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引用次数: 8

Abstract

The present study aims to validate the technique of in vitro ultrasonography (US) by comparative analysis of premortem intima–media thickness (IMT), postmortem IMT and average wall thickness. In vivo common carotid artery (CCA) IMT was measured bilaterally in 25 patients at 30 mm proximal from the flow divider. After autopsy in vitro US was performed and postmortem IMT was measured at the same level. Snap frozen arterial specimens were processed for average wall thickness determination and for histology. High degree of correlation was found between in vivo IMT, in vitro IMT and average wall thickness. Our results demonstrate: (1) in vitro US is a reliable and reproducible tool for the examination of autopsied arterial specimens to obtain valuable information about vascular wall properties and to identify the optimal vascular segment for tissue sampling; (2) snap freezing and cryosectioning of in toto excised arterial specimens is recommended for comparative histological–US studies.

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比较人颈动脉内-中膜厚度的体内和体外死后超声评估和附加的组织学分析

本研究旨在通过对死前内膜-中膜厚度(IMT)、死后内膜-中膜厚度(IMT)和平均壁厚的对比分析，验证体外超声技术(US)的有效性。在离血流分离器近30 mm处测量25例患者的双侧颈总动脉(CCA) IMT。尸体解剖后进行体外US和死后IMT测量在相同水平。处理快速冷冻动脉标本进行平均壁厚测定和组织学检查。体内IMT、体外IMT与平均壁厚高度相关。我们的研究结果表明:(1)体外US是一种可靠的、可重复的工具，用于检查尸检动脉标本，以获得有关血管壁特性的有价值信息，并确定组织采样的最佳血管段;(2)建议对全部切除的动脉标本进行快速冷冻和冷冻切片，用于比较组织学-超声研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Perspectives in Medicine

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