Genetic studies on artemisia (Artemisia monosperma ) and Jatropha (Jatropha curcas) plants by using RAPD technical

Abstract

In the present study, two of the Egyptian Artemisia monosperma and two Jatropha curcas genotypes were selected from Egypt, for genetics analysis by using four RAPD markers. The results indicated that the RAPD primers were able to amplify DNA fragments showing, polymorphic DNA amplification patterns among the genotypes. The data indicated that out of the total 134 bands, 57 were polymorphic (57%). The number of the observed bands ranged from two for the primer j.OPA_07, a.OPA_07 to one in the primer j.OPA_07, a.OPA_07 with an average of 2.3 across for the tested genotypes. Consequently, most of the two RAPD loci in this study were useful for the evaluation of genetic diversity between the two Egyptian Artemisia monosperma , Jatropha genotypes. However, it is worth mentioning, this value expresses the existence to different alleles of one or more loci of homologous chromosomes. Polymorphism data content (PIC) value of the Artemisia, Jatropha genomic RAPD indicated temperate to high level of in formativeness with average PIC value to 0.23 over all the tested RAPD loci primers, where it ranged from 0.29 by using to aOPA-07 to 0.27in jOPA-02 for all genotypes under research by a mean to 0.23. The genetic analysis of the three Artemisia ,Jatropha based on 2 RABD markers detected 53 separate specific alleles, It is noted that, largest number patented to the specific/unique alleles were of the genotype number G2 and G3 where 53 specific alleles were patented to this genotype only.